Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave benzyloxycarbonyl-Arg-thiobenzyl ester (Z-R-SBzl). The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human Tryptase epsilon/BSSP-4 protein
Ala33-Ser317 & Val50-Ser317, both with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ala33 & Val50
Structure / Form
Pro and mature forms
Predicted Molecular Mass
32 kDa
Multiple bands between 33-38 kDa, reducing conditions

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Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 50 mM Tris, 0.05% (w/v) Brij-35, pH 8.0
  • Recombinant Human Tryptase  epsilon /BSSP‑4 (rhBSSP-4) (Catalog # 5047-SE)
  • Substrate: Z-Arg-SBzl (SM Biochemicals, Catalog # SMSB01)
  • DTNB (5,5’-dithibis(2-nitrobenzoic acid)) (Sigma, Catalog # D-8130)
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhBSSP-4 to 0.5 ng/µL in Aassay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer containing 200 µM DTNB.
  3. Load 50 µL of 0.5 ng/µL rhBSSP-4 into a plate, and start the reaction by adding 50 µL of 200 µM Substrate to wells. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
  4. Read at an absorbance of 405 nm for 5 minutes in kinetic mode.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/M
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank

      **Using the extinction coefficient 13260 M-1cm-1

      ***Using the path correction 0.32 cm

     Note: the output of many spectrophotometers is in mOD

Per Well:
  • rhBSSP-4: 0.025 µg
  • Substrate: 100 µM
Reconstitution Calculator

Reconstitution Calculator

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Background: Tryptase epsilon/BSSP-4

Tryptase epsilon, also known as brain-specific protease-4 (BSSP-4) and prosemin, is expressed in the brain and epithelium-rich tissues such as the lung and eye (1, 2). BSSP-4 is a serine protease related to trypsin, preferentially hydrolyzing substrates after arginine and lysine residues. However, Tryptase epsilon is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha 1-antitrypsin and secretory leukocyte protease inhibitor (3). Tryptase  epsilon efficiently converts pro‑urokinase‑type plasminogen activator to its mature, active form (3).

  1. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
  2. Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
  3. Yasuda, S. et al. (2005) Blood 105:3893.
Entrez Gene IDs
64063 (Human); 70835 (Mouse)
Alternate Names
BSP-2; BSSP4; BSSP-4; hBSSP-4; MGC9599; prosemin; protease, serine S1 family member 22; protease, serine, 22; PRSS22; serine protease 22; serine protease 26; SP001LA; Tryptase epsilon; UNQ302/PRO343


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