Tryptase epsilon, also known as brain-specific protease-4 (BSSP-4) and prosemin, is expressed in the brain and epithelium-rich tissues such as the lung and eye (1, 2). BSSP-4 is a serine protease related to trypsin, preferentially hydrolyzing substrates after arginine and lysine residues. However, Tryptase epsilon is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha 1-antitrypsin and secretory leukocyte protease inhibitor (3). Tryptase epsilon efficiently converts pro‑urokinase‑type plasminogen activator to its mature, active form (3).
Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF
R&D Systems | Catalog # 5047-SE
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Key Product Details
- R&D Systems NS0-derived Recombinant Human Tryptase epsilon/BSSP-4 Protein (5047-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Pro and mature forms
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Tryptase epsilon/BSSP-4 protein
Ala33-Ser317 & Val50-Ser317, both with a C-terminal 10-His tag
Ala33-Ser317 & Val50-Ser317, both with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala33 & Val50
Predicted Molecular Mass
32 kDa
SDS-PAGE
Multiple bands between 33-38 kDa, reducing conditions
Activity
Measured by its ability to cleave benzyloxycarbonyl-Arg-thiobenzyl ester (Z-R-SBzl).
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
5047-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tryptase epsilon/BSSP-4
References
- Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
- Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
- Yasuda, S. et al. (2005) Blood 105:3893.
Alternate Names
BSP-2, BSSP-4, BSSP4, PRSS22
Gene Symbol
PRSS22
UniProt
Additional Tryptase epsilon/BSSP-4 Products
Product Documents for Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF (5047-SE):
Materials
- Assay Buffer: 50 mM Tris, 0.05% (w/v) Brij-35, pH 8.0
- Recombinant Human Tryptase epsilon /BSSP‑4 (rhBSSP-4) (Catalog # 5047-SE)
- Substrate: Z-Arg-SBzl (SM Biochemicals, Catalog # SMSB01)
- DTNB (5,5’-dithibis(2-nitrobenzoic acid)) (Sigma, Catalog # D-8130)
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhBSSP-4 to 0.5 ng/µL in Aassay Buffer.
- Dilute Substrate to 200 µM in Assay Buffer containing 200 µM DTNB.
- Load 50 µL of 0.5 ng/µL rhBSSP-4 into a plate, and start the reaction by adding 50 µL of 200 µM Substrate to wells. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
- Read at an absorbance of 405 nm for 5 minutes in kinetic mode.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/M |
| ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Using the extinction coefficient 13260 M-1cm-1
***Using the path correction 0.32 cm
Note: the output of many spectrophotometers is in mOD
Per Well:- rhBSSP-4: 0.025 µg
- Substrate: 100 µM
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