Recombinant Human TSG-6 Protein, CF

Catalog # Availability Size / Price Qty
2104-TS-050
Recombinant Human TSG-6 Protein Binding Activity
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Product Details
Citations (19)
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Recombinant Human TSG-6 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human TSG-6 is present at 0.5 μg/well, the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is found to be approximately 4-30 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human TSG-6 protein
Trp18-Leu277, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Trp18
Predicted Molecular Mass
30.5 kDa
SDS-PAGE
40 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2104-TS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Human TSG-6 Protein Binding Activity View Larger

Recombinant Human TSG-6 (Catalog # 2104-TS) binds hyaluronan in a functional ELISA. When Recombinant Human TSG-6 is present at 0.5 µg/mL, the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is approximately 4-30 ng/mL.

SDS-PAGE Recombinant Human TSG-6 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human TSG-6 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 40 kDa.

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Reconstitution Calculator

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Background: TSG-6

TSG-6 (TNF-stimulated gene 6; also TNFIP6) is a secreted, 35-39 kDa group A member of the LINK‑Module superfamily of proteins (1 - 4). Human TSG‑6 is synthesized as a 277 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 260 aa mature region (5, 6). The mature region shows an N-terminal LINK module (aa 36-129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135-247). Link modules are alpha -helical, beta -sheet structures that bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature human TSG-6 shares 94% aa identity with both mouse and canine TSG-6. Cells reported to express TSG-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral, plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or link, between the individual subunits of extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80-85 kDa HC subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a cation‑dependent catalyst for the removal, transfer, and subsequent covalent linkage of HC 1/2 to surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). The disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

References
  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  6. Lee, T.H. et al. (1992) J. Cell Biol. 116:545.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.
Long Name
Tumor Necrosis Factor-stimulated Gene Sequence 6
Entrez Gene IDs
7130 (Human); 21930 (Mouse)
Alternate Names
Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein

Citations for Recombinant Human TSG-6 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Therapeutic Effects of Human Mesenchymal Stem Cells in a Mouse Model of Cerebellar Ataxia with Neuroinflammation
    Authors: Y Nam, D Yoon, J Hong, MS Kim, TY Lee, KS Kim, HW Lee, K Suk, SR Kim
    J Clin Med, 2020;9(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Anti-inflammatory protein TSG-6 secreted by bone marrow mesenchymal stem cells attenuates neuropathic pain by inhibiting the TLR2/MyD88/NF-&kappaB signaling pathway in spinal microglia
    Authors: H Yang, L Wu, H Deng, Y Chen, H Zhou, M Liu, S Wang, L Zheng, L Zhu, X Lv
    J Neuroinflammation, 2020;17(1):154.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. Intravascular heavy chain-modification of hyaluronan during endotoxic shock
    Authors: K Ni, A Gill, D Cao, K Koike, KS Schweitzer, S Garantziot, I Petrache
    Biochem Biophys Rep, 2019;17(0):114-121.
    Species: Mouse
    Sample Types: Plasma
    Applications: Bioassay
  4. Efficacy of 3D Culture Priming is Maintained in Human Mesenchymal Stem Cells after Extensive Expansion of the Cells
    Authors: TJ Bartosh, JH Ylostalo
    Cells, 2019;8(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Standard
  5. IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance
    Authors: T Muraguchi, D Nanba, EK Nishimura, T Tashiro
    J. Dermatol. Sci., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Bone Marrow Derived Mesenchymal Stromal Cells Ameliorate Ischemia/Reperfusion Injury-Induced Acute Kidney Injury in Rats via Secreting Tumor Necrosis Factor-Inducible Gene 6 Protein
    Authors: Y Chen, X Tang, P Li, Y Zhou, T Xue, J Liu, C Yu
    Biomed Res Int, 2019;2019(0):9845709.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells
    Authors: L Kui, GC Chan, PP Lee
    Mediators Inflamm., 2017;2017(0):7462945.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The role of TSG-6 and uroplakin III in bladder pain syndrome/ interstitial cystitis in rats and humans
    Authors: YS Lv, R Gao, QM Lin, T Jiang, Q Chen, SX Tang, HP Mao, HL Zhou, LS Cao
    Iran J Basic Med Sci, 2017;20(11):1242-1249.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  9. Scalable Production of a Multifunctional Protein (TSG-6) That Aggregates with Itself and the CHO Cells That Synthesize It.
    Authors: Kim D, Choi H, Nishida H, Oh J, Gregory C, Lee R, Yu J, Watanabe J, An S, Bartosh T, Prockop D
    PLoS ONE, 2016;11(1):e0147553.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Intravenous hMSCs Ameliorate Acute Pancreatitis in Mice via Secretion of Tumor Necrosis Factor-? Stimulated Gene/Protein 6
    Sci Rep, 2016;6(0):38438.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. Acute Molecular Changes in Synovial Fluid Following Human Knee Injury: Association With Early Clinical Outcomes
    Authors: Tonia L Vincent
    Arthritis & rheumatology (Hoboken, N.J.), 2016;68(9):2129-40.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  13. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  14. Hyaluronan and Its Heavy Chain Modification in Asthma Severity and Experimental Asthma Exacerbation.
    Authors: Lauer M, Majors A, Comhair S, Ruple L, Matuska B, Subramanian A, Farver C, Dworski R, Grandon D, Laskowski D, Dweik R, Erzurum S, Hascall V, Aronica M
    J Biol Chem, 2015;290(38):23124-34.
    Species: Human
    Sample Types: BALF
    Applications: Bioassay
  15. Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan.
    Authors: Lamkin E, Cheng G, Calabro A, Hascall V, Joo E, Li L, Linhardt R, Lauer M
    J Biol Chem, 2015;290(8):5156-66.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  16. Mesenchymal stromal (stem) cells suppress pro-inflammatory cytokine production but fail to improve survival in experimental staphylococcal toxic shock syndrome.
    Authors: Kim H, Darwish I, Monroy M, Prockop D, Liles W, Kain K
    BMC Immunol, 2014;15(0):1.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6.
    Authors: Lauer M, Glant T, Mikecz K, DeAngelis P, Haller F, Husni M, Hascall V, Calabro A
    J Biol Chem, 2013;288(1):205-14.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  18. Action at a distance: systemically administered adult stem/progenitor cells (MSCs) reduce inflammatory damage to the cornea without engraftment and primarily by secretion of TNF-alpha stimulated gene/protein 6.
    Authors: Roddy GW, Oh JY, Lee RH, Bartosh TJ, Ylostalo J, Coble K, Rosa RH, Prockop DJ
    Stem Cells, 2011;29(10):1572-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  19. Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-kappaB signaling in resident macrophages.
    Authors: Choi H, Lee RH, Bazhanov N, Oh JY, Prockop DJ
    Blood, 2011;118(2):330-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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