Recombinant Human TSG-6 Protein, CF

(11 citations)   
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human TSG-6 is present at 0.5 μg/well, the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is found to be approximately 4-30 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Trp18-Leu277, with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Trp18
  • Predicted Molecular Mass
    30.5 kDa
  • SDS-PAGE
    40 kDa, reducing conditions
2104-TS
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Binding Activity
Recombinant Human TSG-6 (Catalog # 2104-TS) binds hyaluronan in a functional ELISA. When Recombinant Human TSG-6 is present at 0.5 µg/mL, the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is approximately 4-30 ng/mL.
1 μg/lane of Recombinant Human TSG-6 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 40 kDa.
Background: TSG-6

TSG-6 (TNF-stimulated gene 6; also TNFIP6) is a secreted, 35-39 kDa group A member of the LINK‑Module superfamily of proteins (1 - 4). Human TSG‑6 is synthesized as a 277 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 260 aa mature region (5, 6). The mature region shows an N-terminal LINK module (aa 36-129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135-247). Link modules are alpha -helical, beta -sheet structures that bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature human TSG-6 shares 94% aa identity with both mouse and canine TSG-6. Cells reported to express TSG-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral, plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or link, between the individual subunits of extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80-85 kDa HC subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a cation‑dependent catalyst for the removal, transfer, and subsequent covalent linkage of HC 1/2 to surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). The disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

  • References:
    1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
    2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
    3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
    4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
    5. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
    6. Lee, T.H. et al. (1992) J. Cell Biol. 116:545.
    7. Kohda, D. et al. (1996) Cell 86:767.
    8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
    9. Salustri, A. et al. (2003) Development 131:1577.
    10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
    11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
    12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.
  • Long Name:
    Tumor Necrosis Factor-stimulated Gene Sequence 6
  • Entrez Gene IDs:
    7130 (Human); 21930 (Mouse)
  • Alternate Names:
    Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells
    Authors: L Kui, GC Chan, PP Lee
    Mediators Inflamm., 2017;2017(0):7462945.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Scalable Production of a Multifunctional Protein (TSG-6) That Aggregates with Itself and the CHO Cells That Synthesize It.
    Authors: Kim D, Choi H, Nishida H, Oh J, Gregory C, Lee R, Yu J, Watanabe J, An S, Bartosh T, Prockop D
    PLoS ONE, 2016;11(1):e0147553.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  3. TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  4. Intravenous hMSCs Ameliorate Acute Pancreatitis in Mice via Secretion of Tumor Necrosis Factor-? Stimulated Gene/Protein 6
    Sci Rep, 2016;6(0):38438.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  5. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Standard
  6. Hyaluronan and Its Heavy Chain Modification in Asthma Severity and Experimental Asthma Exacerbation.
    Authors: Lauer M, Majors A, Comhair S, Ruple L, Matuska B, Subramanian A, Farver C, Dworski R, Grandon D, Laskowski D, Dweik R, Erzurum S, Hascall V, Aronica M
    J Biol Chem, 2015;290(38):23124-34.
    Species: Human
    Sample Type: BALF
    Application: Bioassay
  7. Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan.
    Authors: Lamkin E, Cheng G, Calabro A, Hascall V, Joo E, Li L, Linhardt R, Lauer M
    J Biol Chem, 2015;290(8):5156-66.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
  8. Mesenchymal stromal (stem) cells suppress pro-inflammatory cytokine production but fail to improve survival in experimental staphylococcal toxic shock syndrome.
    Authors: Kim H, Darwish I, Monroy M, Prockop D, Liles W, Kain K
    BMC Immunol, 2014;15(0):1.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  9. Irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6.
    Authors: Lauer M, Glant T, Mikecz K, DeAngelis P, Haller F, Husni M, Hascall V, Calabro A
    J Biol Chem, 2013;288(1):205-14.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Bioassay
  10. Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-kappaB signaling in resident macrophages.
    Authors: Choi H, Lee RH, Bazhanov N, Oh JY, Prockop DJ
    Blood, 2011;118(2):330-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  11. Action at a distance: systemically administered adult stem/progenitor cells (MSCs) reduce inflammatory damage to the cornea without engraftment and primarily by secretion of TNF-alpha stimulated gene/protein 6.
    Authors: Roddy GW, Oh JY, Lee RH, Bartosh TJ, Ylostalo J, Coble K, Rosa RH, Prockop DJ
    Stem Cells, 2011;29(10):1572-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
Expand to show all 11 Citations
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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