Recombinant Human TSH alpha/beta Heterodimer Protein

Formulations:
Catalog # Availability Size / Price Qty
4610-TH-010
Product Details
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Reviews (2)

Recombinant Human TSH alpha/beta Heterodimer Protein Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human TSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The ED50 for this effect is 0.25-1.5 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human TSH alpha/beta Heterodimer protein
Human TSH alpha
(Ala25-Ser116)
Accession # P01215
Human TSH beta
(Phe21-Val138)
Accession # P01222 
N-terminusC-terminus
N-terminal Sequence
Analysis
Ala25 (TSH alpha ) & Phe21 (TSH beta )
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
10.2 kDa (TSH alpha ), 13.5 kDa (TSH beta )
SDS-PAGE
15-29 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4610-TH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4610-TH/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSH alpha/beta Heterodimer

TSH (thyroid stimulating hormone), also known as thyrotropin, is a member of the cysteine knot growth factor superfamily (1-4). It is a heterodimer of a 15 kDa unique subunit, TSH beta, with a 14 kDa alpha subunit, CGa (common glycoprotein hormone alpha) that is shared with lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG) (1-4). Beta subunits of the four glycoprotein hormones share 37-43% amino acid (aa) identity. Mature human TSH  beta shares 92%, 90%, 90%, 89%, 89%, 89%, and 88% aa identity with canine, rat, equine, mouse, bovine, porcine, and feline TSH beta, respectively. Mature human CGa shares 69%-73% aa identity with canine, rabbit, rat, mouse, bovine, ovine, porcine, feline and equine CGa. Each subunit forms a cysteine knot structure with three disulfide bridges (1). A loop of the TSH beta subunit, termed a “seat-belt”, wraps around the CGa subunit to stabilize non-covalent association of the subunits, and also confers receptor selectivity (5). Structure and charge of the three N-linked carbohydrate chains influence activity; the most complex forms have lower activity but a longer halflife (1, 5). Bovine and porcine TSH bind human TSH receptors (TSHR) with high affinity (6). The hypothalamic peptide TRH stimulates production and secretion of TSH by thyrotrophs (basophile cells) in the anterior pituitary gland (1). TSH travels to thyroid TSHR to stimulate production of thyroxine (T4) (1). In the tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which completes a feedback loop by inhibiting TSH production (1, 7). Studies in the mouse identify bone marrow as a secondary site of TSH production (8). In bone, TSH signaling through TSHR on osteoblast and osteoclast precursors negatively regulates skeletal remodeling (9, 10). Bone marrow cells that produce TSH may also circulate to the thyroid and appear to modulate thyroid hormone activity in times of immunological stress (7, 11).

References
  1. Szkudlinski, M.W. et al. (2002) Physiol. Rev. 82:473.
  2. Sairam, M.R. and C.H. Li (1977) Can. J. Biochem. 55:755.
  3. Fiddes, J.C. and H.M. Goodman (1979) Nature 281:351.
  4. Wondisford, F.E. et al. (1988) J. Biol. Chem. 263:12538.
  5. Weintraub, B.D. and M.W. Szkudlinski (1999) Thyroid 9:447.
  6. Nunez, M.R. et al. (2004) Thyroid 14:991.
  7. Matsushita, A. et al. (2007) Mol. Endocrinol. 21:865.
  8. Klein, J.R. and H-C. Wang (2004) J. Exp. Biol 207:55.
  9. Abe, E. et al. (2003) Cell 115:151.
  10. Sun, L. et al. (2006) Ann. N.Y. Acad. Sci. 1068:309.
  11. Klein, J.R. (2006) Exp. Biol. Med. 231:229.
Long Name
Thyroid Stimulating Hormone
Alternate Names
thyroid stimulating hormone beta; Thyrotropin; TSH alpha/beta Heterodimer; TSHB; TSH-B; TSH-BETA

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Reviews for Recombinant Human TSH alpha/beta Heterodimer Protein

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Recombinant Human TSH alpha/beta Heterodimer Protein
By Anonymous on 04/04/2018
Application: Immunoassay Standard

Recombinant Human TSH alpha/beta Heterodimer Protein
By Anonymous on 05/25/2017
Application: Binding assay/Protein-protein interaction