Recombinant Human UbcH10/UBE2C Protein, CF

Catalog # Availability Size / Price Qty
E2-654-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (6)
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Recombinant Human UbcH10/UBE2C Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

Recombinant Human UbcH10/UBE2C is a member of the Ubiquitin­conjugating Enzyme (E2) family that receives Ubiquitin from a Ubiquitin­Activating Enzyme (E1) and subsequently interacts with a Ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial UbcH10/UBE2C concentration of 0.1-­1 μM.

Source
E. coli-derived human UbcH10/UBE2C protein
Accession # O00762
Accession #
Predicted Molecular Mass
20 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-654

Formulation

0.50 mg/ml (25 μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Gycerol (v/v), 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Reconstitution Calculator

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Background: UbcH10/UBE2C

Ubiquitin­conjugating Enzyme H10 (UbcH10), also known as Ubiquitin­conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin­Conjugating Enzymes (E2) and has a predicted molecular weight of 20 kDa. Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C. UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression. UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11­linked ubiquitination, and promotes target protein degradation in conjunction with APC/C, a Ubiquitin Ligase (E3). The catalytic activity of UbcH10/UBE2C is regulated by a conserved N­terminal extension, which mediates E2­E3 interaction. UbcH10/UBE2C is overexpressed in a variety of human cancers, and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression.

References
  1. Dimova, N.V. et al. (2012) Nat. Cell Biol. 14: 168
  2. Hao, Z. et al. (2012) Tumor Biol. 33: 723
  3. Jiang, L. et al. (2008) Brain Res. 1201: 161
  4. Lin, Y. et al. (2002) J. Biol. Chem. 277: 21913
  5. Okamoto, Y. et al. (2003) Cancer Res. 63: 4167
  6. Summers, M.K. et al. (2008) Mol. Cell 31: 544
  7. van Ree, J.H. et al. (2010) J. Cell Biol. 188: 83
  8. Ye, Y. & M. Rape (2009) Nat. Rev. Mol. Cell Biol. 10: 755
Entrez Gene IDs
11065 (Human); 68612 (Mouse); 296368 (Rat)
Alternate Names
cyclin-selective ubiquitin carrier protein; dJ447F3.2; EC 6.3.2.19; UbcH10; UBCH10mitotic-specific ubiquitin-conjugating enzyme; UBE2C; Ubiquitin carrier protein C; ubiquitin carrier protein E2-C; ubiquitin-conjugating enzyme E2 C; ubiquitin-conjugating enzyme E2C; Ubiquitin-protein ligase C

Citations for Recombinant Human UbcH10/UBE2C Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
    Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
    Cell Death & Disease, 2020;11(9):740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitylation
  2. PI5P4K? functions in DTX1-mediated Notch signaling
    Authors: L Zheng, SD Conner
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis
    Authors: Y Kagami, M Ono, K Yoshida
    Sci Rep, 2017;7(1):5583.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  4. Proteasome inhibition enhances the efficacy of volasertib-induced mitotic arrest in AML in vitro and prolongs survival in vivo
    Authors: D Schnerch, J Schüler, M Follo, J Felthaus, D Wider, K Klingner, C Greil, J Duyster, M Engelhardt, R Wäsch
    Oncotarget, 2017;8(13):21153-21166.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis
    Authors: Y Kagami, M Ono, K Yoshida
    Sci Rep, 2017;7(1):5583.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Neddylation E2 UBE2F promotes the survival of lung cancer cells by activating CRL5 to degrade NOXA via the K11 linkage
    Clin Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination

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