Recombinant Human UbcH10/UBE2C Protein, CF

R&D Systems | Catalog # E2-654

 
R&D Systems
Discontinued Product
E2-654 has been discontinued. View all UbcH10/UBE2C products.

Key Product Details

Source

E. coli

Accession Number

O00762

Applications

Bioactivity
View all UbcH10/UBE2C Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UbcH10/UBE2C protein
Accession # O00762

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

20 kDa

Activity

Recombinant Human UbcH10/UBE2C is a member of the Ubiquitin­conjugating Enzyme (E2) family that receives Ubiquitin from a Ubiquitin­Activating Enzyme (E1) and subsequently interacts with a Ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial UbcH10/UBE2C concentration of 0.1-­1 μM.

Formulation, Preparation, and Storage

E2-654
Formulation

0.50 mg/ml (25 μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Gycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UbcH10/UBE2C

Ubiquitin­conjugating Enzyme H10 (UbcH10), also known as Ubiquitin­conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin­Conjugating Enzymes (E2) and has a predicted molecular weight of 20 kDa. Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C. UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression. UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11­linked ubiquitination, and promotes target protein degradation in conjunction with APC/C, a Ubiquitin Ligase (E3). The catalytic activity of UbcH10/UBE2C is regulated by a conserved N­terminal extension, which mediates E2­E3 interaction. UbcH10/UBE2C is overexpressed in a variety of human cancers, and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression.

References

  1. Dimova, N.V. et al. (2012) Nat. Cell Biol. 14: 168
  2. Hao, Z. et al. (2012) Tumor Biol. 33: 723
  3. Jiang, L. et al. (2008) Brain Res. 1201: 161
  4. Lin, Y. et al. (2002) J. Biol. Chem. 277: 21913
  5. Okamoto, Y. et al. (2003) Cancer Res. 63: 4167
  6. Summers, M.K. et al. (2008) Mol. Cell 31: 544
  7. van Ree, J.H. et al. (2010) J. Cell Biol. 188: 83
  8. Ye, Y. & M. Rape (2009) Nat. Rev. Mol. Cell Biol. 10: 755

Alternate Names

UBE2C

Entrez Gene IDs

11065 (Human); 68612 (Mouse); 296368 (Rat)

Gene Symbol

UBE2C

UniProt

O00762 (Human)

Additional UbcH10/UBE2C Products

Product Documents for Recombinant Human UbcH10/UBE2C Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human UbcH10/UBE2C Protein, CF

For research use only

Citations for Recombinant Human UbcH10/UBE2C Protein, CF

Customer Reviews for Recombinant Human UbcH10/UBE2C Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human UbcH10/UBE2C Protein, CF and earn rewards!

Have you used Recombinant Human UbcH10/UBE2C Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes

Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail