Recombinant Human UbcH2/UBE2H Protein, CF

R&D Systems | Catalog # E2-607

 
R&D Systems
Discontinued Product
E2-607 has been discontinued. View all UbcH2/UBE2H products.

Key Product Details

Source

E. coli

Accession Number

P62256

Applications

Enzyme Activity
View all UbcH2/UBE2H Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UbcH2/UBE2H protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

21 kDa

Activity

Recombinant Human UbcH2/UBE2H is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH2/UBE2H concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E2-607
Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl,10% Glycerol (v/v), 1 mM TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UbcH2/UBE2H

Ubiquitin-conjugating Enzyme H2 (UbcH2), also known as Ubiquitin-conjugating Enzyme E2H (UBE2H), is a member of the Ubiquitin-conjugating (E2) enzyme family (1).  The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. Human UbcH2/UBE2H is highly expressed in skeletal and cardiac muscle, shows significant expression levels in kidney and placenta, and is expressed at low levels in liver, brain, lung, and spleen (2). TNF-alpha induces UbcH2/UBE2H expression via NF-kappa B, and SCL/Tal1 upregulates the expression of UbcH2/UBE2H during erythroid differentiation (2,3). Pathologically, a single nucleotide polymorphism within UbcH2/UBE2H is associated with autism (4). Furthermore, UbcH2/UBE2H has been reported as a putative oncogene for liver tumorigenesis (5).

References

  1. Kaiser, P. et al. (1994) J. Biol. Chem. 269:8797.
  2. Li, Y.P. et al. (2003) FASEB J. 17:1048.
  3. Lausen, J. et al. (2010) J. Biol. Chem. 285:5338.
  4. Vourc'h, P. et al. (2003) Psychiatr. Genet. 13:221.
  5. Keng, V.W. et al. (2009) Nat. Biotechnol. 27:264.
 

Alternate Names

E2-14K, E2-20K, UBC8, UBE2H

Entrez Gene IDs

7328 (Human); 22214 (Mouse)

Gene Symbol

UBE2H

UniProt

P62256 (Human)

Additional UbcH2/UBE2H Products

Product Documents for Recombinant Human UbcH2/UBE2H Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human UbcH2/UBE2H Protein, CF

For research use only

Citations for Recombinant Human UbcH2/UBE2H Protein, CF

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail