Ubiquitin-conjugating Enzyme H5b (UbcH5b), also known as Ubiquitin-conjugating Enzyme E2D 2 (UBE2D2), is a widely expressed member of the Ubiquitin-conjugating (E2) enzyme family (1). The protein has a predicted molecular weight of 16.5 kDa. UbcH5b/UBE2D2 localizes to both the nucleus and the cytoplasm (2). The human protein shares 100% and 92% amino acid sequence identity with the mouse and rat orthologs, respectively. This enzyme has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). UbcH5b/UBE2D2 is capable of mediating the formation of Ubiquitin chains linked through Lys11, Lys48, or Lys63 (4). Working with the SCF(Fbxw2) and MDM2/HDM2 Ubiquitin ligases (E3s), UbcH5b/UBE2D2 mediates the ubiquitination and degradation of the transcription factors GCM1 and p53, respectively (5-7). Along with UBE2N/Ubc13, UbcH5b/UBE2D2 may have a role in the endocytosis and endolysosomal degradation of MHC class I molecules (8). Non-proteolytic ubiquitination of TRIM5-alpha by UbcH5b/UBE2D2 has been reported to block HIV reverse transcription (9). Pathologically, UBE2D family members may be critical targets of cadmium during cadmium-induced renal toxicity (10). Additionally, overexpression of UbcH5b/UBE2D2 has been linked to inflammatory bowel disease (11).
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Recombinant Human UbcH5b/UBE2D2 Protein, CF
R&D Systems | Catalog # E2-622
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human UbcH5b/UBE2D2 Protein (E2-622)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human UbcH5b/UBE2D2 protein
Met1 - Met147
Met1 - Met147
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
17 kDa
Activity
Recombinant Human UbcH5b/UBE2D2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5b/UBE2D2 concentration of 0.1-1 μM.
Reviewed Applications
Read 1 review rated 5 using E2-622 in the following applications:
Formulation, Preparation, and Storage
E2-622
| Formulation | Supplied as a solution in HEPES, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UbcH5b/UBE2D2
References
- Jensen, J.P. et al. (1995) J. Biol. Chem. 270:30408.
- Umebayashi, K. et al. (2008) Mol. Biol. Cell 19:3454.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Ordureau, A. et al. (2008) Biochem. J. 409:43.
- Chiang, M.H. et al. (2008) Biol. Reprod. 79:914.
- Saville, M.K. et al. (2004) J. Biol. Chem. 279:42169.
- Iyappan, S. et al. (2010) J. Biol. Chem. 285:33065.
- Duncan, L.M. et al. (2006) EMBO J. 25:1635.
- Yamauchi, K. et al. (2008) FEBS J. 275:1540.
- Tokumoto, M. et al. (2011) J. Toxicol. Sci. 36:191.
- Hetzenecker, A.M. et al. (2012) Digestion 86:34.
Alternate Names
PUBC1, UBC5B, UBE2D2
Gene Symbol
UBE2D2
UniProt
Additional UbcH5b/UBE2D2 Products
Product Documents for Recombinant Human UbcH5b/UBE2D2 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human UbcH5b/UBE2D2 Protein, CF
For research use only
Citations for Recombinant Human UbcH5b/UBE2D2 Protein, CF
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