Recombinant Human UbcH5c/UBE2D3 Protein, CF

Catalog # Availability Size / Price Qty
E2-627-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (43)
FAQs
Reviews (1)

Recombinant Human UbcH5c/UBE2D3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5c/UBE2D3 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH5c/UBE2D3 protein
Accession #
Predicted Molecular Mass
17 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-627

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: UbcH5c/UBE2D3

Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins (1). For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the Nuclear Factor kappaB (NF-kappaB) inhibitor, IkappaB-alpha, and is implicated in NF-kappaB-dependent inflammation (2-4). UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses (5-7).

References
  1. Page, R.C. et al. (2012) Biochemistry 51:4175.
  2. Gonen, H. et al. (1999) J. Biol. Chem. 274:14823.
  3. Shembade, N. et al. (2010) Science 327:1135.
  4. Xia, Z.P. et al. (2009) Nature 461:114.
  5. Zhang, S. et al. (2008) Cell Cycle 7:3399.
  6. Bentley, M.L. et al. (2011) EMBO J. 30:3285.
  7. Polanowska, J. et al. (2006) EMBO J. 25:2178. 
Entrez Gene IDs
7323 (Human); 66105 (Mouse); 81920 (Rat)
Alternate Names
E2(17)KB 3; E2(17)KB3; EC 6.3.2.19; MGC43926; MGC5416; UBC4/5; UBC5C; UbcH5c; UBE2D3; Ubiquitin carrier protein D3; ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D3

Citations for Recombinant Human UbcH5c/UBE2D3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Competitive binding of E3 ligases TRIM26 and WWP2 controls SOX2 in glioblastoma
    Authors: T Mahlokozer, B Patel, H Chen, P Desouza, X Qu, DD Mao, D Hafez, W Yang, R Taiwo, M Paturu, A Salehi, AD Gujar, GP Dunn, N Mosammapar, AA Petti, H Yano, AH Kim
    Nature Communications, 2021;12(1):6321.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling
    Authors: C Shen, A Nayak, LR Neitzel, AA Adams, M Silver-Ise, LM Sawyer, H Benchabane, H Wang, N Bunnag, B Li, DT Wynn, F Yang, M Garcia-Con, CH Williams, S Dakshanamu, CC Hong, NG Ayad, AJ Capobianco, Y Ahmed, E Lee, DJ Robbins
    Nature Communications, 2021;12(1):5263.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  3. Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection
    Authors: EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow
    Nature, 2021;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitylation
  4. CRL4AMBRA1 is a master regulator of D-type cyclins
    Authors: D Simoneschi, G Rona, N Zhou, YT Jeong, S Jiang, G Milletti, AA Arbini, A O'Sullivan, AA Wang, S Nithikasem, S Keegan, Y Siu, V Cianfanell, E Maiani, F Nazio, F Cecconi, F Boccalatte, D Fenyö, DR Jones, L Busino, M Pagano
    Nature, 2021;592(7856):789-793.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  5. Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain
    Authors: S Shukla, W Ying, F Gray, Y Yao, ML Simes, Q Zhao, H Miao, HJ Cho, P González-A, A Winkler, G Lund, T Purohit, E Kim, X Zhang, JM Ray, S He, C Nikolaidis, J Ndoj, J Wang, ? Jaremko, M Jaremko, RJH Ryan, ML Guzman, J Grembecka, T Cierpicki
    Nature Chemical Biology, 2021;17(7):784-793.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  6. Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents
    Authors: M Deng, J Lin, S Nowsheen, T Liu, Y Zhao, PW Villalta, D Sicard, DJ Tschumperl, S Lee, J Kim, Z Lou
    Science Advances, 2020;6(37):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis
    Authors: CR Kim, T Noda, H Kim, G Kim, S Park, Y Na, S Oura, K Shimada, I Bang, JY Ahn, YR Kim, SK Oh, HJ Choi, JS Kim, I Jung, H Lee, Y Okada, M Ikawa, SH Baek
    Cell Rep, 2020;32(4):107950.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  8. CRL4DCAF8 dependent opposing stability control over the chromatin remodeler LSH orchestrates epigenetic dynamics in ferroptosis
    Authors: D Huang, Q Li, X Sun, X Sun, Y Tang, Y Qu, D Liu, T Yu, G Li, T Tong, Y Zhang
    Cell Death and Differentiation, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
  9. Mutual regulation between OGT and XIAP to control colon cancer cell growth and invasion
    Authors: HG Seo, HB Kim, JY Yoon, TH Kweon, YS Park, J Kang, J Jung, S Son, EC Yi, TH Lee, WH Yang, JW Cho
    Cell Death Dis, 2020;11(9):815.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
    Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
    Cell Death & Disease, 2020;11(9):740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitylation
  11. The FBXW7-SHOC2-Raptor Axis Controls the Cross-Talks between the RAS-ERK and mTORC1 Signaling Pathways
    Authors: CM Xie, M Tan, XT Lin, D Wu, Y Jiang, Y Tan, H Li, Y Ma, X Xiong, Y Sun
    Cell Rep, 2019;26(11):3037-3050.e4.
    Applications: Bioassay
  12. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
    Authors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao
    Cell Rep, 2019;28(2):541-553.e4.
    Applications: Bioassay
  13. Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development
    Authors: P Wang, F Yan, Z Li, Y Yu, SE Parnell, Y Xiong
    J. Clin. Invest., 2019;130(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  14. MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent manner
    Authors: B Liu, J Yi, X Yang, L Liu, X Lou, Z Zhang, H Qi, Z Wang, J Zou, WG Zhu, W Gu, J Luo
    Oncogene, 2018;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  15. Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
    Authors: KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES Fischer
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  16. PCGF5 is required for neural differentiation of embryonic stem cells
    Authors: M Yao, X Zhou, J Zhou, S Gong, G Hu, J Li, K Huang, P Lai, G Shi, AP Hutchins, H Sun, H Wang, H Yao
    Nat Commun, 2018;9(1):1463.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  17. The MtDMI2-MtPUB2 negative feedback loop plays a role in nodulation homeostasis
    Authors: J Liu, J Deng, F Zhu, Y Li, Z Lu, P Qin, T Wang, J Dong
    Plant Physiol., 2018;0(0):.
    Species: Plant - Medicago truncatula
    Sample Types: Recombinant Protein
    Applications: Bioassay
  18. Ubiquitination and dynactin regulate TMEPAI lysosomal trafficking
    Authors: S Luo, L Jing, T Zhao, Y Li, Z Liu, A Diao
    Sci Rep, 2017;7(0):42668.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  19. H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis
    Authors: X Zhang, B Li, AH Rezaeian, X Xu, PC Chou, G Jin, F Han, BS Pan, CY Wang, J Long, A Zhang, CY Huang, FJ Tsai, CH Tsai, C Logothetis, HK Lin
    Nat Commun, 2017;8(0):14799.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  20. Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability
    Authors: M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van Loon
    Sci Rep, 2017;7(1):15050.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  21. BAP1 regulates IP3R3-mediated Ca(2+) flux to mitochondria suppressing cell transformation
    Authors: A Bononi, C Giorgi, S Patergnani, D Larson, K Verbruggen, M Tanji, L Pellegrini, V Signorato, F Olivetto, S Pastorino, M Nasu, A Napolitano, G Gaudino, P Morris, G Sakamoto, LK Ferris, A Danese, A Raimondi, C Tacchetti, S Kuchay, HI Pass, EB Affar, H Yang, P Pinton, M Carbone
    Nature, 2017;546(7659):549-553.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  22. Ubiquitylation of the acetyltransferase MOF in Drosophila melanogaster
    Authors: S Schunter, R Villa, V Flynn, JB Heidelberg, AK Classen, P Beli, PB Becker
    PLoS ONE, 2017;12(5):e0177408.
    Species: Worm - Spodoptera frugiperda (Fall Army Worm)
    Sample Types: Protein
    Applications: Bioassay
  23. HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer
    Authors: SY Kim, HJ Kim, HJ Kim, DH Kim, CH Kim, JH Han, HK Byeon, K Lee
    Autophagy, 2017;0(0):1-89.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  24. The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosis
    Authors: Y Xu, Z Zhang, J Li, J Tong, B Cao, P Taylor, X Tang, D Wu, MF Moran, Y Zeng, X Mao
    J Hematol Oncol, 2017;10(1):132.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  25. Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis
    Authors: LT Gou, JY Kang, P Dai, X Wang, F Li, S Zhao, M Zhang, MM Hua, Y Lu, Y Zhu, Z Li, H Chen, LG Wu, D Li, XD Fu, J Li, HJ Shi, MF Liu
    Cell, 2017;0(0):.
    Applications: Bioassay
  26. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry
    Authors: K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K Husnjak
    Nat. Methods, 2017;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  27. The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase Function
    Authors: S He, Y Cao, P Xie, G Dong, L Zhang
    Sci Rep, 2017;7(0):41364.
  28. In vitro characterization of six STUB1 variants in spinocerebellar ataxia 16 reveals altered structural properties for the encoded CHIP proteins
    Authors: Y Pakdaman, M Sanchez-Gu, R Kleppe, S Erdal, HJ Bustad, L Bjørkhaug, K Haugarvoll, C Tzoulis, K Heimdal, PM Knappskog, S Johansson, I Aukrust
    Biosci. Rep., 2017;37(2):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  29. Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis
    Authors: S Wang, J Juan, Z Zhang, Y Du, Y Xu, J Tong, B Cao, MF Moran, Y Zeng, X Mao
    Cell Death Dis, 2017;8(9):e3058.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  30. A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair
    Authors: JR Brickner, JM Soll, PM Lombardi, CB Vågbø, MC Mudge, C Oyeniran, R Rabe, J Jackson, ME Sullender, E Blazosky, AK Byrum, Y Zhao, MA Corbett, J Gécz, M Field, A Vindigni, G Slupphaug, C Wolberger, N Mosammapar
    Nature, 2017;551(7680):389-393.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  31. C-terminus of HSC70-Interacting Protein (CHIP) Inhibits Adipocyte Differentiation via Ubiquitin- and Proteasome-Mediated Degradation of PPAR?
    Authors: JH Kim, S Shin, J Seo, EW Lee, M Jeong, MS Lee, HJ Han, J Song
    Sci Rep, 2017;7(0):40023.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Ubiquitination
  32. RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes
    Elife, 2016;5(0):.
    Applications: Bioassay
  33. Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stress
    Sci Rep, 2016;6(0):36171.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  34. GpDSR7, a Novel E3 Ubiquitin Ligase Gene in Grimmia pilifera Is Involved in Tolerance to Drought Stress in Arabidopsis
    PLoS ONE, 2016;11(5):e0155455.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  35. TRAF6 Restricts p53 Mitochondrial Translocation, Apoptosis, and Tumor Suppression
    Authors: Hui-Kuan Lin
    Mol. Cell, 2016;64(4):803-814.
    Applications: Bioassay
  36. Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
    Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
    Nat Commun, 2015;6(0):6353.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  37. Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
    Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
    Nat Commun, 2015;6(0):6156.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  38. Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception.
    Authors: Yue, Shen, Tang, Liu-Ya, Tang, Ying, Tang, Yi, Shen, Qiu-Hong, Ding, Jie, Chen, Yan, Zhang, Zengdi, Yu, Ting-Tin, Zhang, Ying E, Cheng, Steven Y
    Elife, 2014;3(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  39. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6.
    Authors: Rodrigo-Brenni M, Gutierrez E, Hegde R
    Mol Cell, 2014;55(2):227-37.
    Applications: Bioassay
  40. Suppression of PPARgamma through MKRN1-mediated ubiquitination and degradation prevents adipocyte differentiation.
    Authors: Kim J, Park K, Lee E, Jang W, Seo J, Shin S, Hwang K, Song J
    Cell Death Differ, 2014;21(4):594-603.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  41. ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.
    Authors: Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou Y
    Mol Cell, 2013;49(6):1108-20.
  42. A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity.
    Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay L
    J Biol Chem, 2012;287(52):43410-6.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  43. Characterization of ML-IAP protein stability and physiological role in vivo.
    Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
    Biochem J, 2012;447(3):427-36.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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Recombinant Human UbcH5c/UBE2D3 Protein, CF
By Anonymous on 01/27/2020
Application: Enzymatic activity in vitro