Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins (1). Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme (2,3). The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression (4-5). UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family (6-9).
Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF
R&D Systems | Catalog # E2-656
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein (E2-656)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived UBE2M/Ubc12 protein
Met1 - Lys183
Met1 - Lys183
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
21 kDa
Activity
Recombinant Human UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2M/Ubc12 concentration of 0.1-1 μM.
Formulation, Preparation, and Storage
E2-656
| Formulation | Supplied as a solution in HEPES, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UBE2M/Ubc12
References
- Gong, L. et al. (1999) J. Biol. Chem. 274:12036.
- Huang, D.T. et al. (2005) Mol. Cell 17:341.
- Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
- Osaka, F. et al. (2000) EMBO J. 19:3475.
- Tateishi, K. et al. (2001) J. Cell Biol. 155:571.
- Huang, A.M. et al. (2011) Biochem. Pharmacol. 82:35.
- Miller, B.W. et al. (2009) Mol. Syst. Biol. 5:315.
- Huang, D.T. et al. (2009) Mol. Cell 33:483.
- Tanaka, T. et al. (2012) Mol. Oncol. 6:267.
Alternate Names
UBC-RS2, UBC12, UbcH12
Gene Symbol
UBE2M
UniProt
Additional UBE2M/Ubc12 Products
Product Documents for Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF
For research use only
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Citations for Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein, CF
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Associated Pathways
