Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF

Catalog # Availability Size / Price Qty
E-305-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (100)
FAQs
Reviews (2)

Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein
Accession #
Predicted Molecular Mass
118 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-305

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin-activating Enzyme/UBE1

Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa.  It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating  – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.   

References
  1. Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
  2. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
  3. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
  4. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  5. Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
  6. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
  7. Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329.
  8. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
  9. Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
  10. Moundry, P. et al. (2012) Cell Cycle 11:1573.
  11. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.
Entrez Gene IDs
7317 (Human); 22201 (Mouse); 314432 (Rat); 100009225 (Rabbit); 853670 (Yeast)
Alternate Names
A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; GXP1; MGC4781; POC20 centriolar protein homolog; POC20; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1A1S9; UBE1X; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing); Ubiquitin-activating enzyme E1; Ubiquitinactivating Enzyme; Ubiquitin-activating Enzyme; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1

Citations for Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

100 Citations: Showing 1 - 10
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  1. Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes
    Authors: S Yoon, K Bogdanov, D Wallach
    Cell Death and Differentiation, 2022;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain
    Authors: S Shukla, W Ying, F Gray, Y Yao, ML Simes, Q Zhao, H Miao, HJ Cho, P González-A, A Winkler, G Lund, T Purohit, E Kim, X Zhang, JM Ray, S He, C Nikolaidis, J Ndoj, J Wang, ? Jaremko, M Jaremko, RJH Ryan, ML Guzman, J Grembecka, T Cierpicki
    Nature Chemical Biology, 2021;17(7):784-793.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  3. BRCA1-BARD1 regulates transcription through BRD4 in Xenopus nucleoplasmic extract
    Authors: JK Barrows, G Fullbright, DT Long
    Nucleic Acids Research, 2021;0(0):.
    Species: N/A
    Sample Types: Plasmid
    Applications: Ubiquitination
  4. Competitive binding of E3 ligases TRIM26 and WWP2 controls SOX2 in glioblastoma
    Authors: T Mahlokozer, B Patel, H Chen, P Desouza, X Qu, DD Mao, D Hafez, W Yang, R Taiwo, M Paturu, A Salehi, AD Gujar, GP Dunn, N Mosammapar, AA Petti, H Yano, AH Kim
    Nature Communications, 2021;12(1):6321.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-kappaB
    Authors: M Won, KA Park, S Kim, E Ju, Y Ko, H Yoo, H Ro, J Lee, J Oh, EG Lee, SY Kim, SW Nam, HM Shen, MK Yeo, JM Kim, GM Hur
    Cell Death and Differentiation, 2021;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. DAZAP2 acts as specifier of the p53 response to DNA damage
    Authors: MC Liebl, J Moehlenbri, H Becker, G Raddatz, SK Abdeen, RI Aqeilan, F Lyko, TG Hofmann
    Nucleic Acids Research, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  7. SARS-CoV-2 Nsp5 Demonstrates Two Distinct Mechanisms Targeting RIG-I and MAVS To Evade the Innate Immune Response
    Authors: Y Liu, C Qin, Y Rao, C Ngo, JJ Feng, J Zhao, S Zhang, TY Wang, J Carriere, AC Savas, M Zarinfar, S Rice, H Yang, W Yuan, JA Camarero, J Yu, XS Chen, C Zhang, P Feng
    MBio, 2021;0(0):e0233521.
    Species: Escherichia coli
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling
    Authors: C Shen, A Nayak, LR Neitzel, AA Adams, M Silver-Ise, LM Sawyer, H Benchabane, H Wang, N Bunnag, B Li, DT Wynn, F Yang, M Garcia-Con, CH Williams, S Dakshanamu, CC Hong, NG Ayad, AJ Capobianco, Y Ahmed, E Lee, DJ Robbins
    Nature Communications, 2021;12(1):5263.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  9. Structural basis of human transcription-DNA repair coupling
    Authors: G Kokic, FR Wagner, A Chernev, H Urlaub, P Cramer
    Nature, 2021;0(0):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  10. FBXL20 promotes breast cancer malignancy by inhibiting apoptosis through degradation of PUMA and BAX
    Authors: RK Manne, Y Agrawal, SK Malonia, S Banday, S Edachery, A Patel, A Kumar, P Shetty, MK Santra
    The Journal of Biological Chemistry, 2021;0(0):101253.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  11. CRL4AMBRA1 is a master regulator of D-type cyclins
    Authors: D Simoneschi, G Rona, N Zhou, YT Jeong, S Jiang, G Milletti, AA Arbini, A O'Sullivan, AA Wang, S Nithikasem, S Keegan, Y Siu, V Cianfanell, E Maiani, F Nazio, F Cecconi, F Boccalatte, D Fenyö, DR Jones, L Busino, M Pagano
    Nature, 2021;592(7856):789-793.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  12. A high-throughput genome-wide RNAi screen identifies modifiers of survival motor neuron protein
    Authors: NM McCormack, MB Abera, ES Arnold, RM Gibbs, SE Martin, E Buehler, YC Chen, L Chen, KH Fischbeck, BG Burnett
    Cell Reports, 2021;35(6):109125.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  13. Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
    Authors: CW Davies, SE Vidal, L Phu, J Sudhamsu, TB Hinkle, S Chan Rosen, FR Schumacher, YJ Zeng, C Schwerdtfe, AS Peterson, JR Lill, CM Rose, AS Shaw, IE Wertz, DS Kirkpatric, JT Koerber
    Nature Communications, 2021;12(1):4608.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  14. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation
    Authors: Y Sun, J Chen, SN Huang, YP Su, W Wang, K Agama, S Saha, LM Jenkins, JM Pascal, Y Pommier
    Nature Communications, 2021;12(1):5010.
    Applications: Bioassay
  15. CRL4DCAF8 dependent opposing stability control over the chromatin remodeler LSH orchestrates epigenetic dynamics in ferroptosis
    Authors: D Huang, Q Li, X Sun, X Sun, Y Tang, Y Qu, D Liu, T Yu, G Li, T Tong, Y Zhang
    Cell Death and Differentiation, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
    Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
    Cell Death & Disease, 2020;11(9):740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitylation
  17. The Ubiquitin Ligase TRIP12 Limits PARP1 Trapping and Constrains PARP Inhibitor Efficiency
    Authors: M Gatti, R Imhof, Q Huang, M Baudis, M Altmeyer
    Cell Rep, 2020;32(5):107985.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  18. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response
    Authors: F Liang, AS Miller, S Longerich, C Tang, D Maranon, EA Williamson, R Hromas, C Wiese, GM Kupfer, P Sung
    Nat Commun, 2019;10(1):2849.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  19. Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development
    Authors: P Wang, F Yan, Z Li, Y Yu, SE Parnell, Y Xiong
    J. Clin. Invest., 2019;130(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  20. Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15
    Authors: TC Ting, M Goralski, K Klein, B Wang, J Kim, Y Xie, D Nijhawan
    Cell Rep, 2019;29(6):1499-1510.e6.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  21. The finger loop of the SRA domain in the E3 ligase UHRF1 is a regulator of ubiquitin targeting and is required for maintaining�DNA methylation
    Authors: RM Vaughan, SB Rothbart, BM Dickson
    J. Biol. Chem., 2019;0(0):.
    Species: Human
    Sample Types: Polynucleosomes
    Applications: Bioassay
  22. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
    Authors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao
    Cell Rep, 2019;28(2):541-553.e4.
    Applications: Bioassay
  23. Mechanism for recycling tRNAs on stalled ribosomes
    Authors: MCJ Yip, AFA Keszei, Q Feng, V Chu, MJ McKenna, S Shao
    Nat. Struct. Mol. Biol., 2019;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  24. K27-linked ubiquitination of BRAF by ITCH engages cytokine response to maintain MEK-ERK signaling
    Authors: Q Yin, T Han, B Fang, G Zhang, C Zhang, ER Roberts, V Izumi, M Zheng, S Jiang, X Yin, M Kim, J Cai, EB Haura, JM Koomen, KSM Smalley, L Wan
    Nat Commun, 2019;10(1):1870.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  25. Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation
    Authors: G Lu, J Yi, A Gubas, YT Wang, Y Wu, Y Ren, M Wu, Y Shi, C Ouyang, HWS Tan, T Wang, L Wang, ND Yang, S Deng, D Xia, RH Chen, SA Tooze, HM Shen
    Autophagy, 2019;0(0):1-18.
    Applications: Bioassay
  26. Binding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination
    Authors: Z Liang, F Liang, Y Teng, X Chen, J Liu, S Longerich, T Rao, AM Green, NB Collins, Y Xiong, L Lan, P Sung, GM Kupfer
    Cell Rep, 2019;26(3):564-572.e5.
    Species: N/A
    Sample Types: DNA
    Applications: Bioassay
  27. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains
    Authors: M Di Rienzo, M Antonioli, C Fusco, Y Liu, M Mari, I Orhon, G Refolo, F Germani, M Corazzari, A Romagnoli, F Ciccosanti, B Mandriani, MT Pellico, R De La Torr, H Ding, M Dentice, M Neri, A Ferlini, F Reggiori, M Kulesz-Mar, M Piacentini, G Merla, GM Fimia
    Sci Adv, 2019;5(5):eaau8857.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  28. Human papillomavirus E7 oncoprotein targets RNF168 to hijack the host DNA damage response
    Authors: J Sitz, SA Blanchet, SF Gameiro, E Biquand, TM Morgan, M Galloy, J Dessapt, EG Lavoie, A Blondeau, BC Smith, JS Mymryk, CA Moody, A Fradet-Tur
    Proc. Natl. Acad. Sci. U.S.A., 2019;116(39):19552-19562.
    Species: Virus - HPV
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  29. Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesis
    Authors: YS Yoo, YJ Park, HS Lee, NTK Oanh, MY Cho, J Heo, ES Lee, H Cho, YY Park, H Cho
    Cell Death Dis, 2019;10(12):938.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  30. E3 ubiquitin ligase RNF123 targets lamin B1 and lamin-binding proteins
    Authors: R Khanna, V Krishnamoo, VK Parnaik
    FEBS J., 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  31. SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate
    Authors: ME Matyskiela, S Couto, X Zheng, G Lu, J Hui, K Stamp, C Drew, Y Ren, M Wang, A Carpenter, CW Lee, T Clayton, W Fang, CC Lu, M Riley, P Abdubek, K Blease, J Hartke, G Kumar, R Vessey, M Rolfe, LG Hamann, PP Chamberlai
    Nat. Chem. Biol., 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  32. The E3 ubiquitin ligase TRIM25 regulates adipocyte differentiation via proteasome-mediated degradation of PPAR?
    Authors: JM Lee, SS Choi, YH Lee, KW Khim, S Yoon, BG Kim, D Nam, PG Suh, K Myung, JH Choi
    Exp. Mol. Med., 2018;50(10):135.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  33. Post-translational modification of OCT4 in breast cancer tumorigenesis
    Authors: Y Cho, HG Kang, SJ Kim, S Lee, S Jee, SG Ahn, MJ Kang, JS Song, JY Chung, EC Yi, KH Chun
    Cell Death Differ., 2018;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  34. MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent manner
    Authors: B Liu, J Yi, X Yang, L Liu, X Lou, Z Zhang, H Qi, Z Wang, J Zou, WG Zhu, W Gu, J Luo
    Oncogene, 2018;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  35. PI5P4K? functions in DTX1-mediated Notch signaling
    Authors: L Zheng, SD Conner
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  36. Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome
    Authors: Y Dong, S Zhang, Z Wu, X Li, WL Wang, Y Zhu, S Stoilova-M, Y Lu, D Finley, Y Mao
    Nature, 2018;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  37. Inhibition of UVSSA ubiquitination suppresses transcription-coupled nucleotide excision repair deficiency caused by dissociation from USP7
    Authors: M Higa, K Tanaka, M Saijo
    FEBS J., 2018;0(0):.
    Applications: Bioassay
  38. The E3 ubiquitin ligase Siah-1 suppresses avian reovirus infection by targeting p10 for degradation
    Authors: X Chen, Z He, M Fu, Y Wang, H Wu, X Li, H Cao, SJ Zheng
    J. Virol., 2018;0(0):.
    Applications: Bioassay
  39. XIAP facilitates breast and colon carcinoma growth via promotion of p62 depletion through ubiquitination-dependent proteasomal degradation
    Authors: X Huang, XN Wang, XD Yuan, WY Wu, PE Lobie, Z Wu
    Oncogene, 2018;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  40. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells
    Authors: S Ito, A Ueno, T Ueda, H Nakagawa, H Taniguchi, N Kayukawa, A Fujihara-I, F Hongo, K Okihara, O Ukimura
    Oncotarget, 2018;9(25):17645-17655.
    Applications: Bioassay
  41. Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast
    Authors: GS Hewawasam, K Dhatchinam, M Mattingly, C Seidel, JL Gerton
    Nucleic Acids Res., 2018;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  42. The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation
    Authors: F Lampert, D Stafa, A Goga, MV Soste, S Gilberto, N Olieric, P Picotti, M Stoffel, M Peter
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  43. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination
    Authors: S Inano, K Sato, Y Katsuki, W Kobayashi, H Tanaka, K Nakajima, S Nakada, H Miyoshi, K Knies, A Takaori-Ko, D Schindler, M Ishiai, H Kurumizaka, M Takata
    Mol. Cell, 2017;66(5):622-634.e8.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  44. CHIP as a membrane-shuttling proteostasis sensor
    Authors: Y Kopp, WH Lang, TB Schuster, A Martínez-L, HF Hofbauer, R Ernst, G Calloni, RM Vabulas
    Elife, 2017;6(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  45. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation
    Authors: X Wei, X Wang, J Zhan, Y Chen, W Fang, L Zhang, H Zhang
    J. Cell Biol., 2017;0(0):.
    Applications: Bioassay
  46. Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlying photomorphogenic development in Arabidopsis
    Authors: X Xu, PK Kathare, VN Pham, Q Bu, A Nguyen, E Huq
    Development, 2017;0(0):.
    Applications: Bioassay
  47. C-terminus of HSC70-Interacting Protein (CHIP) Inhibits Adipocyte Differentiation via Ubiquitin- and Proteasome-Mediated Degradation of PPAR?
    Authors: JH Kim, S Shin, J Seo, EW Lee, M Jeong, MS Lee, HJ Han, J Song
    Sci Rep, 2017;7(0):40023.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Ubiquitination
  48. COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation
    Authors: N Lian, X Liu, X Wang, Y Zhou, H Li, J Li, T Mao
    Proc. Natl. Acad. Sci. U.S.A., 2017;114(46):12321-12326.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  49. BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance
    Authors: B Zhang, M Holmlund, S Lorrain, M Norberg, L Bakó, C Fankhauser, O Nilsson
    Elife, 2017;6(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  50. Tissue-specific Ubiquitination by IPA1 INTERACTING PROTEIN 1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice
    Authors: J Wang, H Yu, G Xiong, Z Lu, Y Jiao, X Meng, G Liu, X Chen, Y Wang, J Li
    Plant Cell, 2017;0(0):.
    Applications: Bioassay
  51. The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress Response
    Authors: ZQ Fan, JY Chen, JF Kuang, WJ Lu, W Shan
    Front Plant Sci, 2017;8(0):995.
    Applications: Bioassay
  52. HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer
    Authors: SY Kim, HJ Kim, HJ Kim, DH Kim, CH Kim, JH Han, HK Byeon, K Lee
    Autophagy, 2017;0(0):1-89.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  53. BAP1 regulates IP3R3-mediated Ca(2+) flux to mitochondria suppressing cell transformation
    Authors: A Bononi, C Giorgi, S Patergnani, D Larson, K Verbruggen, M Tanji, L Pellegrini, V Signorato, F Olivetto, S Pastorino, M Nasu, A Napolitano, G Gaudino, P Morris, G Sakamoto, LK Ferris, A Danese, A Raimondi, C Tacchetti, S Kuchay, HI Pass, EB Affar, H Yang, P Pinton, M Carbone
    Nature, 2017;546(7659):549-553.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  54. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
    Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
    Cell Death Dis, 2017;8(5):e2816.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  55. Ubiquitin-dependent regulation of Cdc42 by XIAP
    Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
    Cell Death Dis, 2017;8(6):e2900.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  56. A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair
    Authors: JR Brickner, JM Soll, PM Lombardi, CB Vågbø, MC Mudge, C Oyeniran, R Rabe, J Jackson, ME Sullender, E Blazosky, AK Byrum, Y Zhao, MA Corbett, J Gécz, M Field, A Vindigni, G Slupphaug, C Wolberger, N Mosammapar
    Nature, 2017;551(7680):389-393.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  57. Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis
    Authors: S Wang, J Juan, Z Zhang, Y Du, Y Xu, J Tong, B Cao, MF Moran, Y Zeng, X Mao
    Cell Death Dis, 2017;8(9):e3058.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  58. Opposing effects of cancer-type-specific SPOP mutants on BET protein degradation and sensitivity to BET inhibitors
    Authors: H Janouskova, G El Tekle, E Bellini, ND Udeshi, A Rinaldi, A Ulbricht, T Bernasocch, G Civenni, M Losa, T Svinkina, CM Bielski, GV Kryukov, L Cascione, S Napoli, RI Enchev, DG Mutch, ME Carney, A Berchuck, BJN Winterhoff, RR Broaddus, P Schraml, H Moch, F Bertoni, CV Catapano, M Peter, SA Carr, LA Garraway, PJ Wild, JP Theurillat
    Nat. Med., 2017;23(9):1046-1054.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  59. Ubiquitylation of the acetyltransferase MOF in Drosophila melanogaster
    Authors: S Schunter, R Villa, V Flynn, JB Heidelberg, AK Classen, P Beli, PB Becker
    PLoS ONE, 2017;12(5):e0177408.
    Species: Worm - Spodoptera frugiperda (Fall Army Worm)
    Sample Types: Protein
    Applications: Bioassay
  60. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
    Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
    Cell Death Dis, 2017;8(5):e2816.
    Species: Bacteria - E. Coli
    Sample Types: Protein
    Applications: Bioassay
  61. Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity
    Authors: Q Ma, H Ruan, L Peng, M Zhang, MU Gack, WD Yao
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  62. Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis Thaliana By Promoting Nuclear Proteasome-Mediated HY5 Degradation
    Authors: C Perea-Resa, MA Rodríguez-, E Iniesto, V Rubio, J Salinas
    Mol Plant, 2017;0(0):.
    Applications: Bioassay
  63. 14-3-3? loss leads to activation of the epithelial to mesenchymal transition due to the stabilization of c-Jun
    Authors: Kumarkrishna Raychaudhu
    J Biol Chem, 2016;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  64. Ubiquitination independent of E1 and E2 enzymes by bacterial effectors
    Authors: J Qiu, MJ Sheedlo, K Yu, Y Tan, ES Nakayasu, C Das, X Liu, ZQ Luo
    Nature, 2016;533(7601):120-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes
    Elife, 2016;5(0):.
    Applications: Bioassay
  66. The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53
    PLoS Biol., 2016;14(11):e2000733.
    Applications: Bioassay
  67. hnRNP E1 Protects Chromosomal Integrity by Translational Regulation of Cdc27
    Authors: LA Link, BV Howley, GS Hussey, PH Howe
    Mol Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Bioassay
  68. SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer
    Oncogene, 2016;0(0):.
    Applications: Bioassay
  69. Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stress
    Sci Rep, 2016;6(0):36171.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  70. Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity
    Nucleic Acids Res., 2016;0(0):.
    Applications: Bioassay
  71. UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 Ubiquitin Ligase
    Authors: Andrea Braganza
    J. Biol. Chem, 2016;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  72. Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation
    Authors: Francesca Nazio
    J. Cell Biol, 2016;215(6):841-856.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  73. TRIM65-catalized ubiquitination is essential for MDA5-mediated antiviral innate immunity
    Authors: Xueting Lang
    J. Exp. Med, 2016;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  74. Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death.
    Authors: Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, Han P, Oh Y
    Cell Death Differ, 2016;23(2):333-46.
    Species: N/A
    Sample Types: Protein
    Applications: Enzyme Assay
  75. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice
    Authors: H Zhang, H Liu, Y Chen, X Yang, P Wang, T Liu, M Deng, B Qin, C Correia, S Lee, J Kim, M Sparks, AA Nair, DL Evans, KR Kalari, P Zhang, L Wang, Z You, SH Kaufmann, Z Lou, H Pei
    Nat Commun, 2016;7(0):10201.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  76. The San1 ubiquitin ligase functions preferentially with ubiquitin-conjugating enzyme Ubc1 during protein quality control
    J Biol Chem, 2016;0(0):.
    Applications: Bioassay
  77. A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase
    Authors: Mary E Matyskiela
    Nature, 2016;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  78. Enzymatic Activity of the Scaffold Protein Rapsyn for Synapse Formation
    Authors: Lin Mei
    Neuron, 2016;92(5):1007-1019.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  79. Cell cycle-regulated ubiquitination of tankyrase 1 by RNF8 and ABRO1/BRCC36 controls the timing of sister telomere resolution
    Authors: Ekta Tripathi
    EMBO J, 2016;0(0):.
    Applications: Bioassay
  80. Identification and Characterization of MCM3 as a Novel KEAP1 Substrate
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  81. Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
    Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
    Nat Commun, 2015;6(0):6353.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  82. Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
    Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
    Nat Commun, 2015;6(0):6156.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  83. Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair.
    Authors: Lee, T-H, Park, J-M, Leem, S-H, Kang, T-H
    Oncogene, 2014;33(1):19-25.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  84. The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2.
    Authors: Townley-Tilson, W H Davi, Wu, Yaxu, Ferguson, James E, Patterson, Cam
    PLoS ONE, 2014;9(2):e89451.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  85. Stochastic gate dynamics regulate the catalytic activity of ubiquitination enzymes.
    Authors: Rout, Manoj K, Hodge, Curtis D, Markin, Craig J, Xu, Xin, Glover, J N Mark, Xiao, Wei, Spyracopoulos, Leo
    J Am Chem Soc, 2014;136(50):17446-58.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  86. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains.
    Authors: Liu, Weixiao, Shang, Yonglian, Zeng, Yan, Liu, Chao, Li, Yanchang, Zhai, Linhui, Wang, Pan, Lou, Jizhong, Xu, Ping, Ye, Yihong, Li, Wei
    EMBO J, 2014;33(1):46-61.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  87. Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception.
    Authors: Yue, Shen, Tang, Liu-Ya, Tang, Ying, Tang, Yi, Shen, Qiu-Hong, Ding, Jie, Chen, Yan, Zhang, Zengdi, Yu, Ting-Tin, Zhang, Ying E, Cheng, Steven Y
    Elife, 2014;3(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  88. Suppression of PPARgamma through MKRN1-mediated ubiquitination and degradation prevents adipocyte differentiation.
    Authors: Kim J, Park K, Lee E, Jang W, Seo J, Shin S, Hwang K, Song J
    Cell Death Differ, 2014;21(4):594-603.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  89. The Rad6/18 ubiquitin complex interacts with the Epstein-Barr virus deubiquitinating enzyme, BPLF1, and contributes to virus infectivity.
    Authors: Kumar, Ravindra, Whitehurst, Christop, Pagano, Joseph S
    J Virol, 2014;88(11):6411-22.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  90. Pin1 modulates ERalpha levels in breast cancer through inhibition of phosphorylation-dependent ubiquitination and degradation.
    Authors: Rajbhandari P, Schalper K, Solodin N, Ellison-Zelski S, Ping Lu K, Rimm D, Alarid E
    Oncogene, 2014;33(11):1438-47.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  91. C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog.
    Authors: Lee, Hyojin, Alpi, Arno F, Park, Mi So, Rose, Ann, Koo, Hyeon-So
    PLoS ONE, 2013;8(3):e60071.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  92. Structural determinants of ubiquitin conjugation in Entamoeba histolytica.
    Authors: Bosch D, Siderovski D
    J Biol Chem, 2013;288(4):2290-302.
    Species: Amoeba - Entamoeba histolytica
    Sample Types: Recombinant Protein
    Applications: Bioassay
  93. A pathogen type III effector with a novel E3 ubiquitin ligase architecture.
    Authors: Singer, Alexande, Schulze, Sebastia, Skarina, Tatiana, Xu, Xiaohui, Cui, Hong, Eschen-Lippold, Lennart, Egler, Monique, Srikumar, Tharan, Raught, Brian, Lee, Justin, Scheel, Dierk, Savchenko, Alexei, Bonas, Ulla
    PLoS Pathog, 2013;9(1):e1003121.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  94. Ubiquitination increases parkin activity to promote autophagic alpha-synuclein clearance.
    Authors: Lonskaya I, Desforges N, Hebron M, Moussa C
    PLoS ONE, 2013;8(12):e83914.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  95. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
    Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
    FASEB J, 2013;28(4):1938-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  96. Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.
    Authors: Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, Cartozo N, Li M, Jager S, Mason-Herr J, Hayashi F, Yokoyama S, Krogan N, Harris R, Peterlin B, Gross J
    PLoS Pathog, 2012;8(12):e1003085.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  97. DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylation regulates p53 transcriptional activation.
    Authors: Pelisch F, Pozzi B, Risso G, Munoz M, Srebrow A
    J Biol Chem, 2012;287(36):30789-99.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  98. TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members.
    Authors: Bell JL, Malyukova A, Holien JK
    PLoS ONE, 2012;7(5):e37470.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  99. Characterization of ML-IAP protein stability and physiological role in vivo.
    Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
    Biochem J, 2012;447(3):427-36.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  100. A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity.
    Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay L
    J Biol Chem, 2012;287(52):43410-6.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination

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Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
By Anonymous on 10/20/2017
Application: Enzymatic activity in vitro

Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
By Anonymous on 08/17/2016
Application: Enzymatic activity in vitro