Recombinant Human VSTM2A His-tag Protein, CF

Catalog # Availability Size / Price Qty
10037-VT-050
Recombinant Human VSTM2A His-tag Protein, CF Bioactivity
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human VSTM2A His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-10 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human VSTM2A protein
Ser25-Phe244, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser25
Predicted Molecular Mass
25 kDa
SDS-PAGE
38-42 kDa, reducing conditions

Product Datasheets

10037-VT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human VSTM2A His-tag Protein, CF Bioactivity View Larger

Recombinant Human VSTM2A (Catalog # 10037-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence ofanti-CD3 antibody. The ED50 for this effect is 1-10 μg/mL.

SDS-PAGE Recombinant Human VSTM2A His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human VSTM2A was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 38-42 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: VSTM2A

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. VSTM2A plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. It promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 3). In humans, two isoforms (1 and 2) exist due to alternative splicing. Human VSTM2A is synthesized either as a 236 amino acid (aa) (Isoform 1) or a 244 aa (Isoform 2) precursor that contains a 24 aa signal sequence followed by the VSTM2A domain. Human Isoform 2 VSTM2A shares 78% aa sequence identity with mouse and rat VSTM2A. Our in house data show that VSTM2A inhibits the human T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion, and T cell proliferation.

References
  1. Secco, B. et al. (2017) Cell Rep. 18:93.
  2. Pandey, A.K. et al. (2014) PloS One. 9:e88889.
  3. Berry, D.C. et al. (2013) Development. 140:3939.
Long Name
V-Set And Transmembrane Domain Containing 2A
Entrez Gene IDs
222008 (Human); 211739 (Mouse); 689106 (Rat)
Alternate Names
MGC33530; V-set and transmembrane domain containing 2; V-set and transmembrane domain containing 2A; V-set and transmembrane domain-containing protein 2A; VSTM2; VSTM2A

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

Reviews for Recombinant Human VSTM2A His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human VSTM2A His-tag Protein, CF and earn rewards!

Have you used Recombinant Human VSTM2A His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review