Recombinant Human VSTM4 Fc Chimera Protein, CF Summary
Recombinant Human VSTM4 Fc Chimera (Catalog # 2086-VT) inhibits IFN-gamma secretion byhuman peripheral blood mononuclear cells in the presence of anti-CD3 antibody. TheED50 for this effect is 1-10 μg/mL.
2 μg/lane of Recombinant Human VSTM4 (Catalog # 2086-VT) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 58-74 kDa and 120-150 kDa, respectively.
V-set and transmembrane domain-containing protein 4 (VSTM4) is a single-pass type I membrane protein in the immunoglobulin superfamily. Human VSTM4 is synthesized as a 320 amino acid (aa) precursor that contains a 23 aa signal sequence, 157 aa extracellular region, 21 aa TM domain, and 119 aa cytoplasmic tail. In humans, part of the extracellular region is cleaved into a 50 aa secreted peptide (aa 55-104) compared to mouse, which is cleaved into a 49 aa peptide (aa 55-103) (1). Because of its role in enhancing L-type voltage-gated calcium channel (L-VGCC) currents in photoreceptors, this peptide was named peptide Lv (1). Peptide Lv is expressed in the central nervous system and a variety of organs including spleen, intestine, retina, and lung (1, 2). The peptide may have possible roles in regulating the cardiovascular system and L-VGCC dependent neural plasticity (1, 2). Human VSTM4 gene is located on chromosome 10, which may be linked to late-onset Alzheimer's disease (3). Down-regulation of VSTM4 increased tamoxifen sensitivity and suppressed growth in cultured breast cancer cells (4). Within the ECD, human VSTM4 shares 87% and 85% aa sequence identity with mouse and rat VSTM4, respectively. The biological functions of VSTM4 remain unknown. Our in-house data show that VSTM4 inhibits the human T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion, and T cell proliferation.
- Shi, L. et al. (2012) PLoS. 7:e43091.
- Shi, L. et al. (2015) Biochim. Biophys. Acta. 1853:1154.
- Grupe, A. et al. (2006) Am. J. Hum. Genet. 78:78.
- Mendes-Pereira, A. et al. (2012) Proc. Natl. Acad. Sci. 109:2730.
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