Recombinant Human YOD1 Protein, CF

R&D Systems | Catalog # E-572

R&D Systems
Discontinued Product
E-572 has been discontinued. View all YOD1 products.

Key Product Details

Source

E. coli

Accession Number

Q5VVQ6

Applications

Bioactivity
View all YOD1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human YOD1 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

39 kDa

Activity

Recombinant Human YOD1 is a Ubiquitin­-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial Recombinant Human YOD1 concentration of 0.1-1 μM.

Reviewed Applications

Read 1 review rated 4 using E-572 in the following applications:

Formulation, Preparation, and Storage

E-572
Formulation 0.77 mg/ml (20 µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: YOD1

OTU1 (Ubiquitin thioesterase OTU1, also known as YOD1) is a deubiquitinating enzyme of the ovarian tumor (otubain, also known as "Otu") family. YOD1 is part of a multi-protein complex containing p97, suggesting this deubiquitinase may function in the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. A possible role for YOD1 is the trimming of polyubiquitin chains from substrates to facilitate their threading through the VCP/p97 pore. YOD1 is capable of hydrolyzing K6-, K11-, K27-, K29-, and K33-linked polyubiquitin chains.

 

 

 

 

 

References

  1. Balakirev M.Y. et al. (2003) EMBO Rep. 4:517-522.
  2. Ernst R. et al. (2009) Mol. Cell 36:28-38.
  3. Komander D. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:550-563.
  4. Messick TE. et al. (2008) J Biol Chem. 283:11038-11049.

Long Name

YOD1 OTU Deubiquinating Enzyme 1 Homolog

Alternate Names

DUBA8, HIN-7, OTUD2

Entrez Gene IDs

55432 (Human); 226418 (Mouse); 363982 (Rat)

Gene Symbol

YOD1

UniProt

Q5VVQ6 (Human)

Additional YOD1 Products

Product Documents for Recombinant Human YOD1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human YOD1 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human YOD1 Protein, CF

Customer Reviews for Recombinant Human YOD1 Protein, CF (1)

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    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/21/2021

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail