Recombinant Human YOD1 Protein, CF

Catalog # Availability Size / Price Qty
E-572-050
Product Details
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Recombinant Human YOD1 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

Recombinant Human YOD1 is a Ubiquitin­-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial Recombinant Human YOD1 concentration of 0.1-1 μM.

Source
E. coli-derived human YOD1 protein
Accession #
Predicted Molecular Mass
39 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-572

Formulation 0.77 mg/ml (20 µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: YOD1

OTU1 (Ubiquitin thioesterase OTU1, also known as YOD1) is a deubiquitinating enzyme of the ovarian tumor (otubain, also known as "Otu") family. YOD1 is part of a multi-protein complex containing p97, suggesting this deubiquitinase may function in the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. A possible role for YOD1 is the trimming of polyubiquitin chains from substrates to facilitate their threading through the VCP/p97 pore. YOD1 is capable of hydrolyzing K6-, K11-, K27-, K29-, and K33-linked polyubiquitin chains.

 

 

 

 

 

References
  1. Balakirev M.Y. et al. (2003) EMBO Rep. 4:517-522.
  2. Ernst R. et al. (2009) Mol. Cell 36:28-38.
  3. Komander D. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:550-563.
  4. Messick TE. et al. (2008) J Biol Chem. 283:11038-11049.
Long Name
YOD1 OTU Deubiquinating Enzyme 1 Homolog
Entrez Gene IDs
55432 (Human); 226418 (Mouse); 363982 (Rat)
Alternate Names
DKFZp451J1719; DUBA8; DUBA-8; DUBA8hsHIN7; EC 3.1.2; EC 3.4.19.12; HIN7; HIN-7; HIV-1-induced protease 7; HsHIN7; OTU domain-containing protein 2; OTUD2; OTUD2OTU domain containing 2; PRO0907; ubiquitin thioesterase OTU1; YOD1 OTU deubiquinating enzyme 1 homolog ( yeast); YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae); YOD1

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