Recombinant Human ZNRF3 Fc Chimera Protein, CF
Recombinant Human ZNRF3 Fc Chimera Protein, CF Summary
Accession # Q9ULT6
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
ZNRF3 (zinc/RING finger protein 3), also called RNF203, is a 101 kDa (predicted) type I transmembrane protein that is a member of the ZNRF3 family of ubiquitin ligases (1). Human ZNRF3 is synthesized as a 936 amino acid (aa) protein that contains a putative 55 aa signal sequence, a 164 aa extracellular domain (ECD), a transmembrane domain, and a cytoplasmic domain with an atypical RING (really interesting new gene) -type zinc finger (1). The mature human ZNRF3 ECD shares 98% aa sequence identity with mouse ZNRF3. An alternative start site at Met101 creates a 90 kDa (predicted) form; the shorter ECD of this human isoform shares 100% aa sequence identity with canine, feline and equine, 99% with porcine, and 98% with rat ZNRF3 (1). In the plasma membrane, ZNRF3 is co‑expressed with the related protein RNF43, and both serve as E3 ubiquitin ligases that promote turnover of Frizzled Wnt receptors and LRP6 to antagonize Wnts (2‑4). Interaction with the R‑Spondin Wnt‑regulating proteins antagonizes ZNRF3, thus stabilizing Frizzleds and LRP6, allowing their accumulation, and promoting Wnt signaling (2, 3). ZNRF3 is expressed in stem cells at the bottom of colon crypts, where it limits the ability of Wnts to induce proliferation (4). It is also expressed in normal gastric tissues, but is down‑regulated in gastric adenocarcinomas (5).
- SwissProt Accession # Q9ULT6.
- MacDonald, B.T. and X. He (2012) Cell Res. 22:1410.
- Hao, H.X. et al. (2012) Nature 485:195.
- Koo, B.K. et al. (2012) Nature 488:665.
- Zhou, Y. et al. (2013) J. Mol. Histol. Mar 17 [Epub ahead of print].
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