Recombinant Monkeypox Virus A30L His-tag Protein, CF

Recombinant Monkeypox Virus A30L His-tag Protein, CF Summary

• R&D Systems HEK293-derived Recombinant Monkeypox Virus A30L His-tag Protein (11282-MX)
• Quality control testing to verify active proteins with lot specific assays by in-house scientists
• All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: A30L

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32 kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37 kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14 kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36 kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2, 3). The A30L subunit is 146 amino acids long and one of the two envelope proteins responsible for allowing for viral entry into a host cell and starting cell to cell fusion (4). The A30L subunit has been the focus of multiple studies for vaccination purposes for not only monkeypox, but for smallpox as well (4). The A30L subunit has also been looked into for development in detection kits for MPXV (5).