Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF

R&D Systems | Catalog # 11263-MX

His-tag, Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein (11263-MX)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_536532.1
View all E8L Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 E8L protein
Met1-Thr275, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

33 kDa

SDS-PAGE

40-46 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF

Recombinant Monkeypox Virus Zaire-96-I-16 E8L His-tag Protein SEC-MALS.

Recombinant Monkeypox Virus Zaire-96-I-16 E8L His-tag Protein (Catalog # 11263-MX) has a molecular weight (MW) of 40.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Monkeypox Virus Zaire-96-I-16 E8L His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Monkeypox Virus Zaire-96-I-16 E8L His-tag Protein (Catalog # 11263-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-46 kDa.

Formulation, Preparation, and Storage

11263-MX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

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Background: E8L

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3).The E8L subunit in MPXV is a surface binding protein that plays a key role in viral entry (4). This happens with E8L binding to chondroitin sulfate on the surface of cells, giving MPXV virion attachment to the host cell. In infected samples, E8L is identified in abundance with intracellular proteins (5).  When targeting the E8L subunit, transmission and replication was reduced by 78% when treated with 100 nM of small interference RNA (siRNA) and reduced by 95% when treated with 200 nM of siRNA (4). The E8L subunit is the most promising target for a newer, more effective vaccine against MPXV, as it has been found to contain human-foreign pentapeptides that could contain epitopes that would be viable for MPXV vaccinations (4, 6-7).

References

  1. Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
  2. Farahat, R.A. et al. (2022) Infez Med. 30:372.
  3. Schelkunov, S.N. et al. (2002) Virology 297:172.
  4. Alkhalil, A. et al. (2009) Virology Journal 6:1.
  5. Brown, J.N. et al. (2010) Mol Cell Proteomics 9:2760.
  6. Focosi, D. et al. (2022)Reviews in Medical Virology: e2392.
  7. Gao, A. et al. (2022) Research Square: DOI: https://doi.org/10.21203/rs.3.rs-1693979/v1.

Long Name

Cell Surface-Binding Protein (MPXV-E8L)

Alternate Names

MPXV-E8L, OPG120

Entrez Gene IDs

929017 (Monkeypox Virus Zaire-96-I-16)

Gene Symbol

OPG120

UniProt

NP_536532.1

Additional E8L Products

Product Documents for Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF

For research use only

Related Research Areas

Serine Proteases and Regulators

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