Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF

Catalog #: 11263-MX Datasheet / COA / SDS

His-tag, Analyzed by SEC-MALS

Catalog #	Availability	Size / Price	Qty
11263-MX-100

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Recombinant Monkeypox Virus Zaire-96-I-16 E8L His-tag Protein SEC-MALS.

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein, CF Summary

R&D Systems HEK293-derived Recombinant Monkeypox Virus Zaire-96-I-16 E8L Protein (11263-MX)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
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Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 E8L protein
Met1-Thr275, with a C-terminal 6-His tag

Accession #

NP_536532.1

N-terminal Sequence
Analysis

Met1

Predicted Molecular Mass

33 kDa

SDS-PAGE

40-46 kDa, under reducing conditions.

Product Datasheets

11263-MX

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11263-MX

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS

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Recombinant Monkeypox Virus Zaire-96-I-16 E8L His-tag Protein (Catalog # 11263-MX) has a molecular weight (MW) of 40.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

SDS-PAGE

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2 μg/lane of Recombinant Monkeypox Virus Zaire-96-I-16 E8L His-tag Protein (Catalog # 11263-MX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-46 kDa.

Reconstitution Calculator

Background: E8L

Monkeypox Virus (MPXV), the virus that causes monkeypox infection in both humans and animals, is a double-stranded DNA virus that has had a recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of viruses (2). It consists of several key subunits including a surface membrane fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ~14 kDa and H3L ~32kDa), an envelope glycoprotein, A35R ~15 kDa), a receptor glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L, ~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa) (2-3).The E8L subunit in MPXV is a surface binding protein that plays a key role in viral entry (4). This happens with E8L binding to chondroitin sulfate on the surface of cells, giving MPXV virion attachment to the host cell. In infected samples, E8L is identified in abundance with intracellular proteins (5). When targeting the E8L subunit, transmission and replication was reduced by 78% when treated with 100 nM of small interference RNA (siRNA) and reduced by 95% when treated with 200 nM of siRNA (4). The E8L subunit is the most promising target for a newer, more effective vaccine against MPXV, as it has been found to contain human-foreign pentapeptides that could contain epitopes that would be viable for MPXV vaccinations (4, 6-7).

References

Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
Farahat, R.A. et al. (2022) Infez Med. 30:372.
Schelkunov, S.N. et al. (2002) Virology 297:172.
Alkhalil, A. et al. (2009) Virology Journal 6:1.
Brown, J.N. et al. (2010) Mol Cell Proteomics 9:2760.
Focosi, D. et al. (2022)Reviews in Medical Virology: e2392.
Gao, A. et al. (2022) Research Square: DOI: https://doi.org/10.21203/rs.3.rs-1693979/v1.