Cathepsin C (CTSC) is a cysteine protease of the papain family (1). It sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro region and a mature region, which are further cleaved during activation to remove the prodomain and split the catalytic domain into heavy and light chains. The N-terminal domain of the pro region is also called the exclusion domain, which remains connected to the heavy chain through non-covalent bonds to the mature, active enzyme. Broadly distributed, CTSC plays a major role in lysosomal degradation and enzyme activation. For example, it activates granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2).
Recombinant Mouse Active Cathepsin C/DPPI Protein, CF
R&D Systems | Catalog # 2336-CY
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Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Active Cathepsin C/DPPI Protein (2336-CY)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Active form
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Cathepsin C/DPPI protein
proform (Asp25-Leu462 with a C-terminal 10-His tag)
The proform was activated by Recombinant Human Cathepsin L (Catalog # 952-CY) and further purified.
proform (Asp25-Leu462 with a C-terminal 10-His tag)
The proform was activated by Recombinant Human Cathepsin L (Catalog # 952-CY) and further purified.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp25 (Exclusion domain) and Asp394 (Light chain)
Predicted Molecular Mass
13 kDa (Exclusion domain), 18 kDa (Heavy chain), and 9 kDa (Light chain)
SDS-PAGE
8 kDa and 19-29 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Arg-7-amido-4-methylcoumarin (GR-AMC).
The specific activity is > 60,000 pmol/min/µg, as measured under the described conditions.
The specific activity is > 60,000 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 2 reviews rated 4.5 using 2336-CY in the following applications:
Formulation, Preparation, and Storage
2336-CY
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin C/DPPI
References
- Turk, et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1192, Academic Press, San Diego.
- Dahl, et al. (2001) Biochemistry 40:1671.
Alternate Names
CTSC, DPPI, PALS, PLS
Gene Symbol
CTSC
UniProt
Additional Cathepsin C/DPPI Products
Product Documents for Recombinant Mouse Active Cathepsin C/DPPI Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Active Cathepsin C/DPPI Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Mouse Active Cathepsin C/DPPI Protein, CF
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Customer Reviews for Recombinant Mouse Active Cathepsin C/DPPI Protein, CF (2)
4.5 out of 5
2 Customer Ratings
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Application: In vivo studyVerified Customer | Posted 12/01/2017
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Protocols
View specific protocols for Recombinant Mouse Active Cathepsin C/DPPI Protein, CF (2336-CY):
Materials
- Assay Buffer: 50 mM MES, 50 mM NaCl, 5 mM DTT, pH 5.5
- Recombinant Mouse Active Cathepsin C/DPPI (rmCathepsin C) (Catalog # 2336-CY)
- Fluorogenic Peptide Substrate: Gly-Arg-AMC (Bachem, Catalog # I-1215), 10 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rmCathepsin C to 0.01 ng/µL in Assay Buffer.
- Dilute Substrate to 800 µM in Assay Buffer.
- Load into a black well plate 50 µL of 0.01 ng/µL rmCathepsin C, and start the reaction by adding 50 µL of 800 µM Substrate. Include a Substrate Blank containing Assay Buffer in place of rmCathepsin C.
- Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A-9891).
Per Well:- rmCathepsin C: 0.0005 µg
- Substrate: 400 µM