Recombinant Mouse Angiopoietin-2 Protein

R&D Systems | Catalog # 7186-AN

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Angiopoietin-2 Protein (7186-AN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_031452

Applications

Bioactivity
View all Angiopoietin-2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Angiopoietin-2 protein
Met1-Phe496, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp68

Predicted Molecular Mass

49.7 kDa

SDS-PAGE

60-66 kDa, reducing conditions

Activity

Measured by its ability to activate Tie-2 in C6 rat glial cells transfected with human Tie-2.
1 μg/mL of Recombinant Mouse Angiopoietin-2 significantly induces phosphorylation of human Tie-2 in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7186-AN
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7186-AN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water. 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-2

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature mouse Ang-2 is 478 amino acids (aa) in length. It contains one coiled‑coil domain (aa 159‑256) that mediates multimerization, and a C‑terminal fibrinogen-like domain (aa 275‑495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang‑2 is 65‑66 kDa in size. Under non‑reducing conditions, both natural and recombinant Ang‑2 form 140 kDa dimers, 200 kDa trimers, and 250‑300 kDa tetramers and pentamers (3‑5). Mature mouse Ang-2 shares 87% and 96% aa sequence identity with human and rat Ang-2, respectively. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1  3, 6). Both Ang‑2 and the related Angiopoietin‑1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang-1 is a potent Tie-2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang‑2 becomes as a Tie-2 agonist (3, 7‑10). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (11, 12). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (7, 12, 13). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang‑1 during lymphatic vessel development (6, 14). It mediates the up‑regulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation (15). Ang-2 is up‑regulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (16‑19). Its direct interaction with Integrins promotes tumor cell invasion (20, 21). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (19).

References

  1. Augustin, H.G. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:165.
  2. Murdoch, C. et al. (2007) J. Immunol. 178:7405.
  3. Maisonpierre, P.C. et al. (1997) Science 27:55.
  4. Procopio, W.N. et al. (1999) J. Biol. Chem. 274:30196.
  5. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126.
  6. Gale, N.W. et al. (2002) Dev. Cell 3:411.
  7. Yuan, H.T. et al. (2009) Mol. Cell. Biol. 29:2011.
  8. Falcon, B.L. et al. (2009) Am. J. Pathol. 175:2159.
  9. Kim, H-Z. et al. (2009) Biochim. Biophys. Acta 1793:772.
  10. Kim, I. et al. (2001) Cardiovasc. Res. 49:872.
  11. Lobov, I.B. et al. (2002) Proc. Natl. Acad. Sci. 99:11205.
  12. Cao, Y. et al. (2007) Cancer Res. 67:3835.
  13. Nasarre, P. et al. (2009) Cancer Res. 69:1324.
  14. Dellinger, M. et al. (2008) Dev. Biol. 319:309.
  15. Fiedler, U. et al. (2006) Nat. Med. 12:235.
  16. Koga, K. et al. (2001) Cancer Res. 61:6248.
  17. Etoh, T. et al. (2001) Cancer Res. 61:2145.
  18. Tressel, S.L. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1989.
  19. Liu, X.S. et al. (2009) J. Biol. Chem. 284:22680.
  20. Hu, B. et al. (2006) Cancer Res. 66:775.
  21. Imanishi, Y. et al. (2007) Cancer Res. 67:4254.

    Alternate Names

    ANGPT2

    Entrez Gene IDs

    285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)

    Gene Symbol

    ANGPT2

    UniProt

    NP_031452

    Additional Angiopoietin-2 Products

    Product Documents for Recombinant Mouse Angiopoietin-2 Protein

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Download SDS 7186-AN (with carrier)
    Download SDS 7186-AN/CF (carrier free)

    Product Specific Notices for Recombinant Mouse Angiopoietin-2 Protein

    For research use only

    Citations for Recombinant Mouse Angiopoietin-2 Protein

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    FAQs for Recombinant Mouse Angiopoietin-2 Protein

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    • Q: This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

      A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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