Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2, CF

Catalog # Availability Size / Price Qty
9796-AN-050
Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2 Binding Activity
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Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Angiopoietin‑like Protein 2/ANGPTL2 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) that produces 50% of the optimal binding response is 12-96 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 2/ANGPTL2 protein
Mouse ANGPTL2
(Ala260-His493)
Accession # AAD55358
HPGGGSGGGSGGGSHHHHHH
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ala260
Predicted Molecular Mass
29 kDa
SDS-PAGE
28-39 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9796-AN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2 Binding Activity View Larger

When Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9796-AN) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 12-96 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2 is a secreted 56 kDa glycoprotein that contains a N-terminal coiled coil domain and a C-terminal fibrinogen like domain (1). ANGPTL2 is one of the seven members of the Angiopoietin Like family proteins, which are structurally similar to angiopoietins (2). Within mature mouse ANGPTL2, amino acid 260-493 contains the C-terminal fibrinogen like domain, and this domain shares 99% and 100% sequence identity with human and rat homologs, respectively. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6-7). ANGPTL2 also function as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled coil domain (8). Receptor of ANGPTL2 is recently identified as LILRB2, and both the coiled coil domain and fibrinogen like domain are required for optimal binding (9).

References
  1. Kim, I. et al. (1999) J Biol Chem 274:26523.
  2. Santulli, G. et al. (2014) Front Endocrinol 5:4.
  3. Tabata, M. et al. (2009) Cell Metab 10:178.
  4. Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
  5. Kikuchi, R. et al. (2008) Cancer Res 68:5067.
  6. Odagiri, H. et al. (2014) Sci Signal 7:ra7.
  7. Endo, M. et al. (2012) Cancer Res 72:1784.
  8. Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
  9. Deng, M. et al. (2014) blood 124:924.
Entrez Gene IDs
23452 (Human); 26360 (Mouse)
Alternate Names
angiopoietin-like 2; Angiopoietin-like Protein 2; angiopoietin-related protein 2; ANGPTL2; ANGRP2; ARP2HARP; MGC8889

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