Recombinant Mouse APCDD1 Protein, CF

R&D Systems | Catalog # 9981-AP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse APCDD1 Protein (9981-AP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q3U128

Applications

Bioactivity
View all APCDD1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse APCDD1 protein
Ser25-Thr492, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser25 & Leu27

Predicted Molecular Mass

54 kDa

SDS-PAGE

54-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Mouse APCDD1 binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) . The ED50 for the binding is 1-8 µg/mL.

Scientific Data Images for Recombinant Mouse APCDD1 Protein, CF

Recombinant Mouse APCDD1 Protein Binding Activity

Recombinant Mouse APCDD1 Protein Binding Activity

Recombinant Mouse APCDD1 (Catalog # 9981-AP) binds to Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) with an ED50 of 1-8 µg/mL.

Recombinant Mouse APCDD1 Protein SDS-PAGE

Recombinant Mouse APCDD1 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse APCDD1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-65 kDa, respectively.

Formulation, Preparation, and Storage

9981-AP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APCDD1

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature mouse APCDD1 consists of a 466 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane domain, and a 1 aa cytoplasmic region. Mouse APCDD1 shares a 98% and 94% amino acid sequence similarity with rat and human respectively. APCDD1 interacts in vitro with Wnt3A and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-precipitate with recombinant Wnt3A and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt3a and LRP5 at the cell surface.

References

  1. Mazzoni, J. et al. (2017) Neuron. 96:1055.
  2. Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
  3. Shimomura, Y. (2010) Nature 464:1043.
  4. Kandimalla, R. (2017) Oncogenesis 6:e308.
  5. Yutaka Shimomura, et al. (2010) Nature 464:1043.
  6. Sung-Gook CHO, (2017) Oncology Letters 14:4845.
  7. Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.

Long Name

APC Down Regulated 1

Alternate Names

APC Down Regulated 1, DRAPC1, HYPT1

Entrez Gene IDs

147495 (Human); 494504 (Mouse); 689616 (Rat)

Gene Symbol

APCDD1

UniProt

Q3U128

Additional APCDD1 Products

Product Documents for Recombinant Mouse APCDD1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse APCDD1 Protein, CF

For research use only

Related Research Areas

Wnt Inhibitors
Wnt Signaling Modulators

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