Recombinant Mouse APCDD1 Protein, CF Summary
Ser25-Thr492, with a C-terminal 6-His tag
Recombinant Mouse APCDD1 (Catalog # 9981-AP) binds to Biotinylated Recombinant MouseWnt-3a (Catalog # BT1324) with an ED50 of 1‑8 µg/mL.
2 μg/lane of Recombinant Mouse APCDD1 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 54-65 kDa, respectively.
APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature mouse APCDD1 consists of a 466 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane domain, and a 1 aa cytoplasmic region. Mouse APCDD1 shares a 98% and 94% amino acid sequence similarity with rat and human respectively. APCDD1 interacts in vitro with Wnt3A and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-precipitate with recombinant Wnt3A and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt3a and LRP5 at the cell surface.
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- Kandimalla, R. (2017) Oncogenesis 6:e308.
- Yutaka Shimomura, et al. (2010) Nature 464:1043.
- Sung-Gook CHO, (2017) Oncology Letters 14:4845.
- Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.
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