Recombinant Mouse APCDD1 Protein, CF

Catalog # Availability Size / Price Qty
9981-AP-050
Recombinant Mouse APCDD1 Protein, CF Binding Activity
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Recombinant Mouse APCDD1 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Mouse APCDD1 binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) . The ED50 for the binding is 1-8 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse APCDD1 protein
Ser25-Thr492, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser25 & Leu27
Predicted Molecular Mass
54 kDa
SDS-PAGE
54-65 kDa, reducing conditions

Product Datasheets

9981-AP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Mouse APCDD1 Protein, CF Binding Activity View Larger

Recombinant Mouse APCDD1 (Catalog # 9981-AP) binds to Biotinylated Recombinant MouseWnt-3a (Catalog # BT1324) with an ED50 of 1‑8 µg/mL.

SDS-PAGE Recombinant Mouse APCDD1 Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse APCDD1 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 54-65 kDa,  respectively.

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Background: APCDD1

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature mouse APCDD1 consists of a 466 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane domain, and a 1 aa cytoplasmic region. Mouse APCDD1 shares a 98% and 94% amino acid sequence similarity with rat and human respectively. APCDD1 interacts in vitro with Wnt3A and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-precipitate with recombinant Wnt3A and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt3a and LRP5 at the cell surface.

References
  1. Mazzoni, J. et al. (2017) Neuron. 96:1055.
  2. Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
  3. Shimomura, Y. (2010) Nature 464:1043.
  4. Kandimalla, R. (2017) Oncogenesis 6:e308.
  5. Yutaka Shimomura, et al. (2010) Nature 464:1043.
  6. Sung-Gook CHO, (2017) Oncology Letters 14:4845.
  7. Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.
Long Name
APC Down Regulated 1
Entrez Gene IDs
147495 (Human); 494504 (Mouse); 689616 (Rat)
Alternate Names
Adenomatosis polyposis coli down-regulated 1 protein; adenomatosis polyposis coli down-regulated 1; APC Down Regulated 1; APCDD1; B7323; DRAPC1; HYPT1; protein APCDD1

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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