Recombinant Mouse Axl Fc Chimera Protein, CF

R&D Systems | Catalog # 854-AX

R&D Systems
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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Axl Fc Chimera Protein (854-AX)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q80YQ3

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all Axl Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Axl protein
Mouse Axl
(His20-Pro443)
Accession # Q80YQ3		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His20

Predicted Molecular Mass

73.8 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Axl Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Gas6 (Catalog # 986‑GS) that produces 50% optimal binding response is 0.4‑2 ng/mL.

Reviewed Applications

Read 3 reviews rated 4 using 854-AX in the following applications:

Formulation, Preparation, and Storage

854-AX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig‑like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Long Name

Axl Receptor Tyrosine Kinase

Alternate Names

AI323647, ARK, JTK11, Tyro7, UFO

Entrez Gene IDs

558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

Gene Symbol

AXL

UniProt

Q80YQ3

Additional Axl Products

Product Documents for Recombinant Mouse Axl Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Axl Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse Axl Fc Chimera Protein, CF

Customer Reviews for Recombinant Mouse Axl Fc Chimera Protein, CF (3)

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  • Recombinant Mouse Axl Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 03/01/2018
    Recombinant Mouse Axl Fc Chimera Protein, CF 854-AX
  • Recombinant Mouse Axl Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/26/2018
    Recombinant Mouse Axl Fc Chimera Protein, CF 854-AX
  • Recombinant Mouse Axl Fc Chimera Protein, CF
    Name: Juwen DuBois
    Application: In vivo study
    Verified Customer | Posted 06/07/2017

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