BACE-2 is an aspartic protease that shares sequence similarity with BACE-1, the major beta ‑secretase for generation of A beta peptide in neurons (1). However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in A beta peptide production (2‑4). The domain structure of mouse BACE-2 consists of a signal peptide (aa 1‑19), pro (aa 20‑62) and a mature chain (aa 63‑514) that consists of a transmembrane domain and a cytoplasmic tail. The C‑terminal region (aa 463‑514) was replaced by a His tag in the Recombinant Mouse BACE-2 that also contained a His residue at the position of Tyr357. The purified rmBACE-2 started at Ala63, the same residue shown at the N-terminus of the human active BACE-2 (2).
Recombinant Mouse BACE-2 Protein, CF
R&D Systems | Catalog # 2977-AS
Key Product Details
- R&D Systems NS0-derived Recombinant Mouse BACE-2 Protein (2977-AS)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Phe29-Pro462 (Tyr357His) with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010).
The specific activity is >50 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
2977-AS
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution |
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| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BACE-2
References
- Cai, H. et al. (2001) Nature Neurosci. 4:233.
- Hussain, I. et al. (2001) J. Biol. Chem. 276:23322.
- Ostermann, N. et al. (2006) J. Mol. Biol. 355:249.
- Sun, X. et al. (2005) FASEB J. 19:739.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional BACE-2 Products
Product Documents for Recombinant Mouse BACE-2 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse BACE-2 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Mouse BACE-2 Protein, CF
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Protocols
View specific protocols for Recombinant Mouse BACE-2 Protein, CF (2977-AS):
- Assay Buffer: 50 mM NaOAc, 1.0 M NaCl, 0.05% Brij-35 (w/v), pH 3.0
- Recombinant Mouse BACE-2 (rmBACE-2) (Catalog # 2977-AS)
- Substrate: MCA-Lys-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 (Catalog # ES010)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rmBACE-2 to 2 ng/µL in Assay Buffer.
- Dilute Substrate to 20 µM in Assay Buffer.
- Load 50 µL of the 2 ng/µL rmBACE-2 in a plate, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
Per Well:
- rmBACE-2: 0.1 µg
- Substrate: 10 µM