Recombinant Mouse BMP-9 Protein

Formulations:
Catalog # Availability Size / Price Qty
5566-BP-010
Product Details
Citations (7)
FAQs
Reviews

Recombinant Mouse BMP-9 Protein Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.4-1.6 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse BMP-9 protein
Ser319-Arg428
Accession #
N-terminal Sequence
Analysis
Ser319
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.1 kDa
SDS-PAGE
13 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5566-BP

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5566-BP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-9

Bone morphogenetic protein 9 (BMP-9), also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP family of TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). Mouse BMP-9 consists of a 22 amino acid (aa) signal sequence, a 296 aa propeptide, and a 110 aa mature protein. Unlike other BMP family propeptides, the BMP-9 propeptide does not interfere with the biological activity of BMP-9, and remains associated with the mature peptide after proteolytic cleavage (3). Mouse and human BMP-9 share 96% aa sequence identity. Within the mature protein, mouse BMP-9 shares 64% aa sequence identity with mouse BMP-10 and approximately 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver where it promotes lipid metabolism and inhibits glucose production (4 - 6). It exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (6). BMP-9 interacts with the high affinity ALK-1 receptor on liver endothelial cells (3 - 5). It induces a cholinergic neuronal phenotype as well as the differentiation of mesenchymal stem cells into the chondrogenic lineage (7 - 11). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations it enhances TGF-beta 1 production, and inhibits hematopoietic progenitor colony formation (12). BMP-9 also inhibits angiogenesis and the invasiveness of prostate carcinoma cells (13, 14).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
  4. Song, J.J. et al. (1995) Endocrinology 136:4293.
  5. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
  6. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
  7. Lopez-Coviella, I. et al. (2000) Science 289:313.
  8. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984.
  9. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
  10. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
  11. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
  12. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
  13. Ye, L. et al. (2008) Mol. Cancer Res. 6:1594.
  14. David, L. et al. (2008) Circ. Res. 102:914.
Long Name
Bone Morphogenetic Protein 9
Entrez Gene IDs
2658 (Human); 12165 (Mouse)
Alternate Names
BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2

Citations for Recombinant Mouse BMP-9 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. SMAD4 Deficiency Leads to Development of Arteriovenous Malformations in Neonatal and Adult Mice
    Authors: YH Kim, SW Choe, MY Chae, S Hong, SP Oh
    J Am Heart Assoc, 2018;7(21):e009514.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer
    Authors: M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly
    J. Exp. Clin. Cancer Res., 2018;37(1):209.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
    Sci Rep, 2016;5(0):37366.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA (Capture)
  4. Functional and splicing defect analysis of 23 ACVRL1 mutations in a cohort of patients affected by Hereditary Hemorrhagic Telangiectasia.
    Authors: Alaa El Din F, Patri S, Thoreau V, Rodriguez-Ballesteros M, Hamade E, Bailly S, Gilbert-Dussardier B, Abou Merhi R, Kitzis A
    PLoS ONE, 2015;10(7):e0132111.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. BMP-9 regulates the osteoblastic differentiation and calcification of vascular smooth muscle cells through an ALK1 mediated pathway.
    Authors: Zhu D, Mackenzie N, Shanahan C, Shroff R, Farquharson C, MacRae V
    J Cell Mol Med, 2015;19(1):165-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Osteoblast-Specific gamma-Glutamyl Carboxylase-Deficient Mice Display Enhanced Bone Formation With Aberrant Mineralization.
    Authors: Azuma K, Shiba S, Hasegawa T, Ikeda K, Urano T, Horie-Inoue K, Ouchi Y, Amizuka N, Inoue S
    J Bone Miner Res, 2015;30(7):1245-54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
    Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing
    Nat Genet, 2013;45(1):34-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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