Recombinant Mouse BST-2/Tetherin Protein, CF

Catalog # Availability Size / Price Qty
9940-BS-050
Recombinant Mouse BST-2/Tetherin Protein, CF Binding Activity
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Recombinant Mouse BST-2/Tetherin Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse BST-2/Tetherin (Catalog # 9940-BS) is immobilized at 1 µg/mL (100 µL/well), Recombinant Viral Dengue Virus 4 Envelope Fc Chimera binds with an ED50 of 0.2-1.6 μg/mL.
Source
E. coli-derived mouse BST-2/Tetherin protein
Thr52-Ser152, with a C-Terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr52
Predicted Molecular Mass
12 kDa
SDS-PAGE
13 kDa and 28 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9940-BS

Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Mouse BST-2/Tetherin (Catalog # 9940-BS) isimmobilized at 1 µg/mL (100 µL/well), Recombinant Viral Dengue Virus 4 EnvelopeFc Chimera binds with an ED50 of 0.2-1.6 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse BST-2/Tetherin (Catalog # 9940-BS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 13 kDa and 28 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BST-2/Tetherin

BST-2, also known as tetherin or CD317, is a type II transmembrane protein that is constitutively expressed in several cell types including T cells, B cells, monocytes and macrophages, as well as on several cancer cell lines including the B cell lineage in multiple myeloma (1, 2). Originally named bone marrow stromal cell antigen 2, BST-2 was identified to inhibit viral replication due to its unusual topology (3-5). The extracellular C-terminus of the protein is modified with GPI (glycosylphosphatidylinositol) membrane anchor, which enables BST-2 to interact at each of its ends with the cell or the viral membrane. The ectodomain of the protein also contains three cysteine residues that can form intermolecular disulfide bonds to form parallel dimers (2). Mouse BST-2 consists of a 30 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 101 aa extracellular domain (ECD). Within the ECD, mouse BST-2 shares 41% and 71% aa sequence identity with human and rat BST-2, respectively. BST-2 was identified as a major mediator of the innate immune defense against enveloped viruses. BST-2 was shown to interact with formed viral particles tethering them to surface of infected cells, thereby reducing viral release (6). BST-2 inhibits the release of diverse group of enveloped viruses including members of the retrovirus, togavirus, filovirus, arenavirus, flavivirus, rhabdovirus, orthohepadnavirus, orthomyxovirus, poxvirus, paramyxovirus, and herpesvirus families (7-9). The mechanism BST-2 inhibition of viral budding was suggested to be through directly bridging the host and viral membranes through simultaneous embedding of its two opposing membrane anchors (9). To counteract BST-2 functions, many viruses have evolved to develop strategies to antagonize BST-2 function (9, 10). In addition to inhibiting viral release, BST-2 can act as an innate immune sensor of viral infections by acting as a pattern recognition receptor that activates NF-kappa B to induce inflammatory responses through interactions with the immunoglobulin-like transcript 7 (ILT7/LILRA4) (10, 11).

References
  1. Evans, D.T. et al. (2010) Trens Microbiol. 18:388.
  2. Ohtomo, T. et al. (1999) Biochem. Biophys. Res. Commun. 258:583.
  3. Ishikawa, J. et al. (1995) Genomics. 26:527.
  4. Neil, S.J. et al. (2008) Nature. 451:425.
  5. Kupzig, S. et al. (2003) Traffic. 4:694.
  6. Janvier K. et al. (2012) Curr HIV Res. 10:315.
  7. Evans D.T. et al. (2010) Trends Microbiol. 18:388.
  8. Mahauad-Fernandez W.D. et al. (2012) Immun Inflamm Dis. 4:4.
  9. Swiecki M. et al. (2013) Mol Immunol. 54:132.
  10. Sauter D. (2014) Front Microbiol. 5:163.
  11. Hotter D. et al. (2013) J Mol Biol. 425:4956.
Long Name
Bone Marrow Stromal Cell Antigen 2
Entrez Gene IDs
684 (Human); 69550 (Mouse)
Alternate Names
bone marrow stromal antigen 2; bone marrow stromal cell antigen 2; BST2; BST-2; CD317 antigen; CD317; HM1.24 Antigen; NPC-A-7; PDCA-1; Tetherin

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