Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

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Catalog # Availability Size / Price Qty
8997-BT-050
Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF Data
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Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 1-5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse BTN2A2/Butyrophilin 2A2 protein
Gln30-Trp248, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln30 inferred from enzymatic pyroglutamate treatment revealing Phe31
Predicted Molecular Mass
26 kDa
SDS-PAGE
38-45 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8997-BT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Mouse BTN2A2/Butyrophilin 2A2 inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50for this effect is 1-6 ug/mL.

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Background: BTN2A2/Butyrophilin 2A2

Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature mouse Butyrophilin 2A2 consisits of a 215 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 249 aa cytoplasmic domain. Within the Ig-like domains, mouse Butyrophilin 2A2 shares 71% and 90% aa sequence identity with human and rat Butyrophilin 2A2, respectively. Alternative splicing generates an additional isoform of mouse Butyrophilin 2A2 with a deletion and substitution in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3+ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).

References
  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
  3. Ammann, J.U. et al. (2013) J. Immunol. 190:5030.
  4. Sinkus, M.L. et al. (2013) Brain Behav. Immun. 32:51.
Long Name
Butyrophilin Subfamily 2 Member A2
Entrez Gene IDs
10385 (Human); 238555 (Mouse); 306957 (Rat)
Alternate Names
BTF2; BTF2BT2.2butyrophilin 2; BTN2; BTN2A2; Butyrophilin 2; butyrophilin subfamily 2 member A2; butyrophilin, subfamily 2, member A2; FLJ41908

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