Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 1-5 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived mouse BTN2A2/Butyrophilin 2A2 protein
    Gln30-Trp248, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln30 inferred from enzymatic pyroglutamate treatment revealing Phe31
  • Predicted Molecular Mass
    26 kDa
  • SDS-PAGE
    38-45 kDa, reducing conditions
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8997-BT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
 
Reconstitution Reconstitute at 200 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Recombinant Mouse BTN2A2/Butyrophilin 2A2 inhibits IL-2secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 forthis effect is 1-6 ug/mL.
Background: BTN2A2/Butyrophilin 2A2
Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature mouse Butyrophilin 2A2 consisits of a 215 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 249 aa cytoplasmic domain. Within the Ig-like domains, mouse Butyrophilin 2A2 shares 71% and 90% aa sequence identity with human and rat Butyrophilin 2A2, respectively. Alternative splicing generates an additional isoform of mouse Butyrophilin 2A2 with a deletion and substitution in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3+ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).
  • References:
    1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
    2. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
    3. Ammann, J.U. et al. (2013) J. Immunol. 190:5030.
    4. Sinkus, M.L. et al. (2013) Brain Behav. Immun. 32:51.
  • Long Name:
    Butyrophilin Subfamily 2 Member A2
  • Entrez Gene IDs:
    10385 (Human); 238555 (Mouse); 306957 (Rat)
  • Alternate Names:
    BTF2; BTF2BT2.2butyrophilin 2; BTN2; BTN2A2; Butyrophilin 2; butyrophilin subfamily 2 member A2; butyrophilin, subfamily 2, member A2; FLJ41908
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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 31  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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