Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

Learn More About B7-related Butryophilins as Immune Modulators
View Webinar
Catalog # Availability Size / Price Qty
8997-BT-050
Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF Bioactivity
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 1-5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse BTN2A2/Butyrophilin 2A2 protein
Gln30-Trp248, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln30 inferred from enzymatic pyroglutamate treatment revealing Phe31
Predicted Molecular Mass
26 kDa
SDS-PAGE
38-45 kDa, reducing conditions

Product Datasheets

8997-BT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF Bioactivity View Larger

Recombinant Mouse BTN2A2/Butyrophilin 2A2 inhibits IL-2secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 forthis effect is 1-6 ug/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BTN2A2/Butyrophilin 2A2

Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature mouse Butyrophilin 2A2 consisits of a 215 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 249 aa cytoplasmic domain. Within the Ig-like domains, mouse Butyrophilin 2A2 shares 71% and 90% aa sequence identity with human and rat Butyrophilin 2A2, respectively. Alternative splicing generates an additional isoform of mouse Butyrophilin 2A2 with a deletion and substitution in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3+ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).

References
  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
  3. Ammann, J.U. et al. (2013) J. Immunol. 190:5030.
  4. Sinkus, M.L. et al. (2013) Brain Behav. Immun. 32:51.
Long Name
Butyrophilin Subfamily 2 Member A2
Entrez Gene IDs
10385 (Human); 238555 (Mouse); 306957 (Rat)
Alternate Names
BTF2; BTF2BT2.2butyrophilin 2; BTN2; BTN2A2; Butyrophilin 2; butyrophilin subfamily 2 member A2; butyrophilin, subfamily 2, member A2; FLJ41908

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF and earn rewards!

Have you used Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review