Recombinant Mouse Cadherin-6/KCAD Protein, CF

R&D Systems | Catalog # 6826-CA

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Cadherin-6/KCAD Protein (6826-CA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_031692

Applications

Bioactivity
View all Cadherin-6/KCAD Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Cadherin-6/KCAD protein
Met1-Ala615 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn22 (pro-protein) & Ser54 (mature protein)

Predicted Molecular Mass

67.0 kDa (pro-protein) & 63.3 kDa (mature protein)

SDS-PAGE

90-105 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells.
The ED50 for this effect is 0.2-1.0 μg/mL after a 30 minutes incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

6826-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in DPBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cadherin-6/KCAD

Cadherin‑6, also known as KCAD or K‑Cadherin, is a 110‑120 kDa type I transmembrane glycoprotein belonging to the classical Cadherin superfamily of
calcium‑dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Mouse Cadherin‑6 is synthesized with a 18 amino acid (aa) signal peptide and a 35 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 562 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3). Within the ECD, mouse Cadherin‑6 shares 96% aa sequence identity with human and rat Cadherin‑10. It interacts homotypically as well as heterotypically with Cadherin‑9 and more weakly with Cadherins‑7, ‑10, and ‑14 (4, 5). Cadherin‑6 is expressed primarily in the kidney and brain, both during development and in the adult (6‑9). It is enriched in renal proximal tubule epithelial cells and their precursors at sites of cell‑cell contact as well as on basolateral membranes (7, 10, 11). In the nervous system, Cadherin‑6 is neuronally expressed in discrete regions of the neural plate, neural crest cells that give rise to peripheral nerves, and the brain (3, 6). Cadherin‑6 is additionally expressed in the developing retina, osteoclasts and their progenitors, ovarian epithelium, and endometrium during the follicular phase of the menstrual cycle (12‑15). It is also expressed in carcinomas of the kidney, ovary, and liver (8, 10, 11, 14).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Inoue, T. et al. (1997) Dev. Biol. 183:183.
  4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  5. Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
  6. Inoue, T. et al. (1998) Dev. Dyn. 211:338.
  7. Xiang Y.-Y. et al. (1994) Cancer Res. 54:3034.
  8. Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
  9. Cho, E.A. et al. (1998) Development 125:803.
  10. Paul, R. et al. (1997) Cancer Res. 57:2741.
  11. Shimazui, T. et al. (2000) Eur. Urol. 38:331.
  12. Ruan, G. et al. (2006) Mech. Dev. 123:881.
  13. Mbalaviele, G.  et al. (1998) J. Cell Biol.  141:1467.
  14. Sellar, G.C. et al. (2001) Cancer Res.  61:6977.
  15. Getsios, S. et al. (1998) Dev. Dyn. 211:238.

Alternate Names

Cadherin6, CDH6, K-Cadherin

Entrez Gene IDs

1004 (Human)

Gene Symbol

CDH6

UniProt

NP_031692

Additional Cadherin-6/KCAD Products

Product Documents for Recombinant Mouse Cadherin-6/KCAD Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Cadherin-6/KCAD Protein, CF

For research use only

Citations for Recombinant Mouse Cadherin-6/KCAD Protein, CF

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