Carboxylesterase 3 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). These carboxylesterases are widely distributed in mammalian tissues and have broad substrate specificity ranging from small molecule esters such as phenylester to long‑chain fatty acid esters and thioesters. Because many ester-containing drugs require the ester linkage to improve the bioavailability of therapeutic agents, CESs play a major role in drug metabolism and activation. CES3 is also known as triacylglycerol hydrolase for its lipolytic activity. It has been reported to be the major lipase in white adipose tissue (3).
Recombinant Mouse Carboxylesterase 3/CES3 Protein, CF
R&D Systems | Catalog # 5428-CE
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Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Carboxylesterase 3/CES3 Protein (5428-CE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Carboxylesterase 3/CES3 protein
Tyr19-Glu561, with a C-terminal 10-His tag
Tyr19-Glu561, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Tyr19
Predicted Molecular Mass
61 kDa
SDS-PAGE
58 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze p-nitrophenylacetate.
The specific activity is >11,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >11,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
5428-CE
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carboxylesterase 3/CES3
References
- Redinbo, M. R. and Potter, P.M. (2005) Drug Discovery Today. 10:313.
- Satoh, T. and Hosokawa, M. (2006) Chem.-Biol. Interactions. 162:195.
- Soni, K. G. et al. (2004) J. Biol. Chem. 279:40683.
Alternate Names
CES3
Gene Symbol
CES3
UniProt
Additional Carboxylesterase 3/CES3 Products
Product Documents for Recombinant Mouse Carboxylesterase 3/CES3 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Carboxylesterase 3/CES3 Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Mouse Carboxylesterase 3/CES3 Protein, CF (5428-CE):
Materials
- Assay Buffer: 50 mM Tris, pH 7.5
- Recombinant Mouse Carboxylesterase 3/CES3 (rmCES3) (Catalog # 5428-CE)
- Substrate: 4-Nitrophenyl acetate (4-NPA) (Sigma, Catalog # N-8130), 100 mM stock in Acetone
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rmCES3 to 0.2 ng/µL in assay buffer.
- Dilute substrate 4-NPA to 2 mM in deionized water.
- Load in a clear microplate 50 µL of 0.2 ng/µL rmCES3, and start the reaction by adding 50 µL of 2 mM 4-NPA. Include a blank consisting of 50 µL of assay buffer and 50 µL 2 mM 4-NPA.
- Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).
Per Well:- rmCES3: 0.010 µg
- Substrate: 1 mM
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