Recombinant Mouse Carboxylesterase 31-like/EG13909, CF

R&D Systems | Catalog # 6059-CE

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Carboxylesterase 31-like/EG13909 (6059-CE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q8VCU1

Structure / Form

Monomer

Applications

Enzyme Activity
View all Carboxylesterase 31-like/EG13909 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Carboxylesterase 31-like/EG13909 protein
Met1-Glu558, with a C-terminal 10-His tag
Accession # Q8VCU1

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro29

Predicted Molecular Mass

60 kDa

SDS-PAGE

62 kDa, reducing conditions

Activity

Measured by its esterase activity.
The specific activity is >750 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

6059-CE
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Carboxylesterase 31-like/EG13909

EG13909, also known as Es-31, is a carboxylesterase-like enzyme identified in mouse liver (1). It belongs to the type B carboxylesterase/lipase superfamily that hydrolyzes the carboxylic ester bonds. By sequence analysis it is most closely related to mouse CES-3 and is likely to be a microsomal enzyme. It has been suggested that EG13909 is expressed predominantly in male livers and might be regulated by growth hormone (1).

References

  1. Aida, K. et al. (1993) Biochim. Biophys. Acta. 1174:72.

Alternate Names

Carboxylesterase 31 like, EG13909

Entrez Gene IDs

13909 (Mouse)

Gene Symbol

CES3B

UniProt

Q8VCU1

Additional Carboxylesterase 31-like/EG13909 Products

Product Documents for Recombinant Mouse Carboxylesterase 31-like/EG13909, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse Carboxylesterase 31-like/EG13909, CF

For research use only

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Protocols

View specific protocols for Recombinant Mouse Carboxylesterase 31-like/EG13909, CF (6059-CE):

Materials
  • Assay Buffer: 50 mM Tris, pH 7.5
  • Absorbance Buffer: 50 mM Tris, pH 8.0
  • Recombinant Mouse Carboxylesterase 31 like/EG13909 (rmEG13909) (Catalog # 6059-CE)
  • Substrate: 4-Nitrophenyl acetate (4-NPA) (Sigma, Catalog # N8130), 100 mM stock in acetone
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rmEG13909 to 2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 2.0 mM in Assay Buffer.
  3. Load 50 µL of 2 ng/µL rmEG13909 into plate and start the reaction by adding 50 µL of 2.0 mM Substrate. As a Substrate Blank combine 50 µL Assay Buffer with 50 µL Substrate.
  4. Cover and incubate at room temperature for 60 minutes in the dark.
  5. After incubation add 100 µL Absorbance Buffer and read plate immediately.
  6. Read (top read) absorbance in endpoint mode at 410 nm.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard p-Nitrophenol (Sigma-Aldrich, Catalog # 241326).

Per Well:
  • rmEG13909: 0.1 µg
  • Substrate: 0.5 mM

FAQs

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