Recombinant Mouse Cathepsin X/Z/P Protein, CF

R&D Systems | Catalog # 1033-CY

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Cathepsin X/Z/P Protein (1033-CY)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9WUU7

Structure / Form

Pro form

Applications

Enzyme Activity
View all Cathepsin X/Z/P Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Cathepsin X/Z/P protein
Ala23-Val306 & Leu27-Val306, both with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23 & Leu27

Predicted Molecular Mass

33 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >1,200 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation, and Storage

1033-CY
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a relatively new cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References

  1. Deussing, et al. (2000) Biochim. Biophys. Acta 1491:93.
  2. Santamaria, et al. (1998) J. Biol. Chem. 273:16816.
  3. Nagler and Menard (1998) FEBS Lett. 434:135.
  4. Pungercar and Ivanovski (2000) Pflugers Arch. 439:R116.
  5. Pungercar, et al. (2000) Pflugers Arch. 439:R119.
  6. Sivaraman, et al. (2000) J. Mol. Biol. 295:935.
  7. Menard, et al. (2001) Biol. Chem. 382:839.
  8. Therrien, et al. (2001) Biochemistry 40:2702.
  9. Klemencic, et al. (2000) Eur. J. Biochem. 267:5404.
  10. Guncar, et al. (2000) Structure Fold Des. 8:305.
  11. Lustigman, et al. (1996) J. Biol. Chem. 271:30181.

Alternate Names

Cathepsin P, Cathepsin Z, CTSZ

Entrez Gene IDs

1522 (Human); 64138 (Mouse)

Gene Symbol

CTSZ

UniProt

Q9WUU7

Additional Cathepsin X/Z/P Products

Product Documents for Recombinant Mouse Cathepsin X/Z/P Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Cathepsin X/Z/P Protein, CF

For research use only

Citations for Recombinant Mouse Cathepsin X/Z/P Protein, CF

Customer Reviews for Recombinant Mouse Cathepsin X/Z/P Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Cathepsin X/Z/P Protein, CF and earn rewards!

Have you used Recombinant Mouse Cathepsin X/Z/P Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Mouse Cathepsin X/Z/P Protein, CF (1033-CY):

Materials
  • Assay Buffer: 25 mM NaOAc, 5 mM DTT, pH 3.0
  • Recombinant Mouse Cathepsin X/Z/P (rmCathepsin X/Z/P) (Catalog # 1033-CY)
  • Fluorogenic Peptide Substrate V: MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(DNP)-OH (Catalog # ES005)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent

  1. Dilute rmCathepsin X/Z/P to 10 µg/mL in Assay Buffer.
  2. Incubate at room temperature for 5 minutes to activate.
  3. Dilute activated rmCathepsin X/Z/P to 0.4 ng/µL in Assay Buffer.
  4. Dilute Substrate to 20 µM in Assay Buffer.
  5. Load 50 µL of the 0.4 ng/µL rmCathepsin X/Z/P into a black well plate, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate without any rmCathepsin X/Z/P.
  6. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975)

Per Well:

  • rmCathepsin X/Z/P: 0.02 µg
  • Substrate: 10 µM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes