Recombinant Mouse CCL1/TCA-3 Protein

Carrier Free

Catalog # Availability Size / Price Qty
845-TC-025/CF

With Carrier

Catalog # Availability Size / Price Qty
845-TC-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
FAQs
Reviews (2)

Recombinant Mouse CCL1/TCA-3 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR8. The ED50 for this effect is 0.5-2.0 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CCL1/I-309/TCA-3 protein
Lys24-Cys92
Accession #
N-terminal Sequence
Analysis
Lys24
Predicted Molecular Mass
7.8 kDa
SDS-PAGE
13-16 kDa, reducing conditions

Product Datasheets

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845-TC (with carrier)

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845-TC/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

845-TC

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

845-TC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: CCL1/I-309/TCA-3

Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

References
  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.
Entrez Gene IDs
6346 (Human); 20290 (Mouse)
Alternate Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

Citations for Recombinant Mouse CCL1/TCA-3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. CC chemokine CCL1 receptor CCR8 mediates conversion of mesenchymal stem cells to embryoid bodies expressing FOXP3+CCR8+ regulatory T cells
    Authors: NS Haque, A Tuteja, N Haque
    PLoS ONE, 2019-07-17;14(7):e0218944.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Sox2 Communicates With Tregs Through CCL1 To Promote The Stemness Property Of Breast Cancer Cells
    Authors: Y Xu, X Dong, P Qi, Y Ye, W Shen, L Leng, L Wang, X Li, X Luo, Y Chen, P Sun, R Xiang, N Li
    Stem Cells, 2017-12-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
    Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
    J. Immunol, 2016-02-05;196(6):2514-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Mast cell FcepsilonRI-induced early growth response 2 regulates CC chemokine ligand 1-dependent CD4+ T cell migration.
    Authors: Wu Z, MacNeil A, Junkins R, Li B, Berman J, Lin T
    J Immunol, 2013-03-27;190(9):4500-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
    Authors: Islam SA, Chang DS, Colvin RA
    Nat. Immunol., 2011-01-09;12(2):167-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81.
    Applications: ELISA (Standard)
  7. CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
    Authors: Kearley J, Robinson DS, Lloyd CM
    J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6.
    Applications: ELISA (Standard)
  8. Yaba-like disease virus protein 7L is a cell-surface receptor for chemokine CCL1.
    Authors: Najarro P, Lee HJ, Fox J, Pease J, Smith GL
    J. Gen. Virol., 2003-12-01;84(0):3325-36.
    Species: Virus
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse CCL1/TCA-3 Protein

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Mouse CCL1/TCA-3 Protein
By Anonymous on 11/04/2019
Application: Characterization prior to use in downstream assays.
Reason for Rating: SEC elution consistent with expected MW, material appears to be good quality

Recombinant Mouse CCL1/TCA-3 Protein
By Anonymous on 01/05/2018
Application: Immunoassay Standard

The recombinant TCA-3 protein was used as the standard with antibodies MAB8451 and BAF845 in an ELISA to measure mouse samples.