Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 27-119) Protein

Carrier Free

Catalog # Availability Size / Price Qty
1017-MP-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1017-MP-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 27-119) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 1‑5 ng/mL.
Source
E. coli-derived mouse CCL24/Eotaxin-2/MPIF-2 protein
Val27-Val119
Accession #
N-terminal Sequence
Analysis
Val27
Predicted Molecular Mass
10.3 kDa

Product Datasheets

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1017-MP (with carrier)

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1017-MP/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1017-MP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1017-MP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to Eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin. Two amino-terminally-truncated isoforms of mouse Eotaxin-2 have been produced at R&D Systems. The Val27 - Val119 mouse Eotaxin-2 isoform has been shown to be 150-fold more potent than the Ile24 - Val119 isoform as a chemoattractant for mouse BaR/3 cells transfected with mouse CCR3 (1 - 3).

References
  1. Zimmermann, N. et al. (2000) J. Immunol. 165:5839.
  2. Watanabe, K. et al. (2002) J. Immunol. 168:1911.
  3. Grzegorzewski, K.J. et al. (2001) Cytokine 13:209.
Entrez Gene IDs
6369 (Human); 56221 (Mouse)
Alternate Names
CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6

Citation for Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 27-119) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
    Authors: Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O&amp;amp;apos;Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA
    J. Immunol., 2007;178(12):7879-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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