Recombinant Mouse CCL6/C10 Protein

R&D Systems | Catalog # 487-C

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse CCL6/C10 Protein (487-C)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P27784

Applications

Bioactivity
View all CCL6/C10 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse CCL6/C10 protein
Gly22-Ala116

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly22

Predicted Molecular Mass

10.7 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1.
The ED50 for this effect is 50-250 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 487-C in the following applications:

Scientific Data Images for Recombinant Mouse CCL6/C10 Protein

Recombinant Mouse CCL6/C10 Protein Bioactivity

Recombinant Mouse CCL6/C10 Protein Bioactivity

Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts BaF3 mouse pro B cells transfected with human CCR1. The ED50 for this effect is 50-250 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 487-C
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 487-C/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL6/C10

Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11. The E. coli expressed CCL6 produced at R&D Systems has been shown to be a monocyte chemoattractant.

Alternate Names

C10, MRP-1, SCYA6

Entrez Gene IDs

20305 (Mouse); 287910 (Rat)

Gene Symbol

CCL6

UniProt

P27784

Additional CCL6/C10 Products

Product Documents for Recombinant Mouse CCL6/C10 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 487-C (with carrier)
Download SDS 487-C/CF (carrier free)

Product Specific Notices for Recombinant Mouse CCL6/C10 Protein

For research use only

Citations for Recombinant Mouse CCL6/C10 Protein

Customer Reviews for Recombinant Mouse CCL6/C10 Protein (1)

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  • Recombinant Mouse CCL6/C10 Protein
    Name: Patrick Duncker
    Application: Cell migration/motility
    Verified Customer | Posted 12/04/2017
    Recombinant Mouse CCL6/C10 Protein 487-C

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