Recombinant Mouse CCL7/MARC Protein

R&D Systems | Catalog # 456-MC

R&D Systems
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse CCL7/MARC Protein (456-MC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q03366.1

Applications

Bioactivity
View all CCL7/MCP-3/MARC Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse CCL7/MCP-3/MARC protein
Gln24-Pro97

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln24

Predicted Molecular Mass

8.5 kDa

Activity

Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485.
The ED50 for this effect is 0.1-0.3 µg/mL.

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A.
The ED50 for this effect is 0.5‑2 µg/mL.

Reviewed Applications

Read 1 review rated 5 using 456-MC in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 456-MC
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 456-MC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL7/MCP-3/MARC

Mouse MARC, a member of the beta  subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor-activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity. The E. coli-expressed mouse MARC/MCP-3 produced at R&D Systems has been shown to be a monocyte and T-lymphocyte chemoattractant.

References

  1. Kulmburg, P.A. et al. (1992) J. Exp. Med. 176:1773.
  2. Thirion, S. et al. (1994) Biochem. Biophys. Res. Commun. 201:493.
  3. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
  4. Kurihara, T. and R. Bravo (1996) J. Biol. Chem. 271:11603.

Alternate Names

MARC, MCP-3

Entrez Gene IDs

6354 (Human); 20306 (Mouse)

Gene Symbol

CCL7

UniProt

Q03366.1

Additional CCL7/MCP-3/MARC Products

Product Documents for Recombinant Mouse CCL7/MARC Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 456-MC (with carrier)
Download SDS 456-MC/CF (carrier free)

Product Specific Notices for Recombinant Mouse CCL7/MARC Protein

For research use only

Citations for Recombinant Mouse CCL7/MARC Protein

Customer Reviews for Recombinant Mouse CCL7/MARC Protein (1)

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    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/28/2019

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