Recombinant Mouse CD200 His Tagged Protein, CF

R&D Systems | Catalog # 9867-CD

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CD200 His Tagged Protein (9867-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

O54901

Applications

Bioactivity
View all CD200 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD200 protein
Gln31-Gly232, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln31 inferred from enzymatic pyroglutamate treatment revealing Val32

Predicted Molecular Mass

23 kDa

SDS-PAGE

40-52 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD200 R1 Fc Chimera (Catalog # 2554‑CD) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD200 that produces 50% of the optimal binding response is 0.25‑1.5 μg/mL.

Scientific Data Images for Recombinant Mouse CD200 His Tagged Protein, CF

Recombinant Mouse CD200 His Tagged Protein Binding Activity

Recombinant Mouse CD200 His Tagged Protein Binding Activity

When Recombinant Mouse CD200 R1 Fc Chimera Recombinant Mouse CD200 R1 Fc Chimera (Catalog # 2554-CD) is coated at 1 µg/mL, 100 µL/well, Recombinant Mouse CD200 (Catalog # 9867-CD) binds with an ED50 of 0.25-1.5 µg/mL.
Recombinant Mouse CD200 His Tagged Protein SDS-PAGE

Recombinant Mouse CD200 His Tagged Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CD200 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 40-55 kDa.

Formulation, Preparation, and Storage

9867-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD200

CD200, also known as OX-2, is a 45 kDa type I transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The mouse CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-likeV-type and one Ig-like C2-type domain (3). Splice variants of CD200 have been described in human but not in mouse. Within the ECD, mouse CD200 shares 76% and 94% aa sequence identity with human and rat CD200, respectively. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5-7). CD200 knockout mice are characterized by increased macrophage number and activation, and are predisposed to autoimmune disorders (8). CD200 and CD200 R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200 R initiates inhibitory signals following receptor-ligand contact (6, 7, 10). In T cells, CD200 functions as a co-stimulatory molecule that is independent of the CD28 pathway (11). Several additional CD200 R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14-16).

References

  1. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  2. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  3. Chen, Z. et al. (1997) Biochim. Biophys. Acta 1362:6.
  4. Wright, G.J. et al. (2001) Immunology 102:173.
  5. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  7. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  8. Hoek, R.M. et al. (2000) Science 290:1768.
  9. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  10. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  11. Borriello, F. et al. (1997) J. Immunol. 158:4548.
  12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  13. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  14. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
  15. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
  16. Langlais, C.L. et al. (2006) J. Virol. 80:3098.

Alternate Names

CD200, MOX1, MOX2, MRC, OX-2

Entrez Gene IDs

4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)

Gene Symbol

CD200

UniProt

O54901 (Goat)

Additional CD200 Products

Product Documents for Recombinant Mouse CD200 His Tagged Protein, CF

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Product Specific Notices for Recombinant Mouse CD200 His Tagged Protein, CF

For research use only

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