Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2580-LM-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.3-1.8 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse CD300b/LMIR5 protein
Mouse CD300b
(Ile18-Tyr157)
Accession # Q3U497
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ile18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43.2 kDa (monomer)
SDS-PAGE
55-58 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2580-LM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD300b/LMIR5

LMIR5, also known as CD300b, CD300LB, CLM-7, and IREM‑3, is a 26 kDa-32 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse LMIR5 consists of a 140 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, mouse LMIR5 shares 51% and 86% aa sequence identity with human and rat LMIR5, respectively. The transmembrane segment contains a positively charged lysine which enables the association of LMIR5 with DAP12, DAP10, and potentially other adaptor proteins (2, 3). The cytoplasmic domain of human LMIR5 contains a phosphorylatable tyrosine motif, while that of mouse LMIR5 does not (2). LMIR5 is expressed on the surface of myeloid lineage cells (2-4). It forms noncovalent cis‑homodimers and cis-heterodimers with other CD300 family proteins, and the composition of these dimers affects the cellular response (2, 5). Antibody cross‑linking of LMIR5 induces mast cell granule release and cytokine production as well as its tyrosine phosphorylation of LMIR5 (in human) (2, 3). LMIR5 interacts with TIM1 and TIM4 which regulate T cell activation and are themselves binding partners (6). TIM1 interactions with LMIR5 mediate mast cell activation and the accumulation of neutrophils at sites of TIM1 up‑regulation on damaged renal tubule epithelial cells (6).

References
  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Martinez-Barriocanal, A. et al. (2006) J. Immunol. 177:2819.
  3. Yamanishi, Y. et al. (2008) Blood 111:688.
  4. Wu, Y. et al. (2011) Cell. Immunol. 268:68.
  5. Martinez-Barriocanal, A. et al. (2010) J. Biol. Chem. 285:41781.
  6. Yamanishi, Y. et al. (2010) J. Exp. Med. 207:1501.
Long Name
Leukocyte Mono Ig-like Receptor 5
Entrez Gene IDs
124599 (Human); 217304 (Mouse)
Alternate Names
CD300 antigen like family member B; CD300 antigen-like family member B; CD300 molecule-like family member b; CD300b antigen; CD300b; CD300LB; CLM7; CLM-7; CLM7IREM3CD300 molecule like family member b; CMRF35A2; CMRF35-A2; CMRF35-like molecule 7; EC 1.13.11.6; EC 6.3.4.3; Gm253; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; LMIR5; TREM-5; TREM5CD300b; Triggering receptor expressed on myeloid cells 5

Citation for Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A soluble form of LMIR5/CD300b amplifies lipopolysaccharide-induced lethal inflammation in sepsis.
    J. Immunol., 2012;189(4):1773-9.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review