Recombinant Mouse CD300c2 Fc Chimera Protein, CF

R&D Systems | Catalog # 5838-LM

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse CD300c2 Fc Chimera Protein (5838-LM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

BAC80269

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD300c2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse protein
Mouse CD300c2
(Cys22-Ser193)
Accession # BAC80269		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys22

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and serially diluted Recombinant Mouse CD300c2 Fc Chimera.
The ED50 for this effect is 0.6‑3 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

5838-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300c2

Leukocyte mono-Ig-like receptor 2 (LMIR2), also known as CMRF-35A, CD300c2, CLM4, DIgR1, and MAIR-II, is a 35 kDa type I transmembrane glycoprotein that belongs to the immunoregulatory signaling (IRS) family of immunoglobulin-like molecules (1). Mature mouse LMIR2 consists of a 165 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 20 aa cytoplasmic tail (2). An alternate splice form lacks two amino acids C-terminal to the Ig-like domain (3). Within the ECD, mouse LMIR2 shares 45% aa sequence identity with human LMIR2. The CMRF-35 antibody detects both LMIR2 and LMIR1/CMRF-35H which are 92% identical in their Ig-like domains (4-7). LMIR2 is expressed on the surface of subpopulations of bone marrow derived dendritic cells and mast cells, monocytes, peritoneal macrophages, marginal zone B cells, and T cells (2, 3, 6, 8). It contains at least one positively charged amino acid in its transmembrane segment which may mediate LMIR2 association with the adaptor signaling proteins DAP10, DAP12, and FcR gamma (3, 8). Cross-linking of LMIR2 on macrophages induces the secretion of the proinflammatory molecules TNF-alpha, IL-6, and CCL2 (3).

References

  1. Clark, G.J. et al. (2001) Tissue Antigens 57:415.
  2. Luo, K. et al. (2001) Biochem. Biophys. Res. Commun. 287:35.
  3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  4. Jackson, D.G. et al. (1992) Eur. J. Immunol. 22:1157.
  5. Daish, A. et al. (1993) Immunology 79:55.  
  6. Green, B.J. et al. (1998) Int. Immunol. 10:891.
  7. Clark, G.J. et al. (2007) J. Leukoc. Biol. 82:1126.
  8. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.

Long Name

CD300C molecule 2

Alternate Names

CLM4, IGSF7, LMIR2, MAIR-II

Entrez Gene IDs

100131439 (Human); 140497 (Mouse)

Gene Symbol

CD300LD

UniProt

BAC80269

Additional CD300c2 Products

Product Documents for Recombinant Mouse CD300c2 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD300c2 Fc Chimera Protein, CF

For research use only

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