Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
7698-LM-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.75-3.5 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse CD300e/LMIR6 protein
Mouse CD300E
(Leu18-Leu166)
Accession # EDL34457
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43.6 kDa (monomer)
SDS-PAGE
55-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7698-LM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300e/LMIR6

CD300E, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35 kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 154 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 4 aa cytoplasmic stub (2). Within the ECD, mouse CD300E shares 57% and 78% aa sequence identity with human and rat CD300E, respectively. CD300E is expressed on monocytes and dendritic cells (3, 4). Its transmembrane segment contains a charged lysine residue which mediates CD300E association with the signaling adaptor molecule DAP12 and enables CD300E to function as an activating receptor (2, 3). Antibody crosslinking of CD300E promotes monocyte and dendritic cell survival, production of inflammatory chemokines and cytokines, upregulation of costimulatory molecules, and also the ability of dendritic cells to induce T cell proliferation (4).

References
  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Chung, D.H. et al. (2003) J. Immunol. 141:6541.
  3. Aguilar, H. et al. (2004) J. Immunol. 173:6703.
  4. Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.
Entrez Gene IDs
342510 (Human); 217306 (Mouse)
Alternate Names
CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2

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