Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF

  
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.75-3.5 μg/mL.
    Optimal dilutions should be determined by each laboratory for each application.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Mouse CD300E
    (Leu18-Leu166)
    Accession # EDL34457
    IEGRMDP Mouse IgG2A
    (Glu98-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu18
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    43.6 kDa (monomer)
  • SDS-PAGE
    55-60 kDa, reducing conditions
7698-LM
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD300e/LMIR6
CD300E, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35 kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 154 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 4 aa cytoplasmic stub (2). Within the ECD, mouse CD300E shares 57% and 78% aa sequence identity with human and rat CD300E, respectively. CD300E is expressed on monocytes and dendritic cells (3, 4). Its transmembrane segment contains a charged lysine residue which mediates CD300E association with the signaling adaptor molecule DAP12 and enables CD300E to function as an activating receptor (2, 3). Antibody crosslinking of CD300E promotes monocyte and dendritic cell survival, production of inflammatory chemokines and cytokines, upregulation of costimulatory molecules, and also the ability of dendritic cells to induce T cell proliferation (4).
  • References:
    1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
    2. Chung, D.H. et al. (2003) J. Immunol. 141:6541.
    3. Aguilar, H. et al. (2004) J. Immunol. 173:6703.
    4. Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.
  • Entrez Gene IDs:
    342510 (Human); 217306 (Mouse)
  • Alternate Names:
    CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2
Related Research Areas

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