Recombinant Mouse CD5 Protein, CF

R&D Systems | Catalog # 115-CD

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CD5 Protein (115-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P13379

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD5 protein
Gln24-Asn370, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln24 predicted, sequencing might be blocked

Predicted Molecular Mass

39.2 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Zymosan is coated at 200 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD5 that produces 50% optimal binding response is 0.6-3 μg/mL.

Formulation, Preparation, and Storage

115-CD
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD5

CD5, also known as Leu-1, Ly-1, and T1, is a 67 kDa transmembrane glycoprotein in the scavenger receptor superfamily (1). Mature mouse CD5 consists of a 347 amino acid (aa) extracellular domain (ECD) with three scavenger receptor cysteine-rich (SRCR) domains, a 30 aa transmembrane segment, and a 93 aa cytoplasmic domain (2). Within the ECD, mouse CD5 shares 55% and 82% aa sequence identity with human and rat CD5, respectively. The 52 kDa ECD can be cleaved from the cell surface and circulates in the serum (3). CD5 has been shown to interact homophilically, with CD72 on B cells, and with beta-glucan components of fungal cell walls (4-6). CD5 expression on developing thymocytes is positively regulated by signaling through the T cell antigen receptor (TCR) and is up‑regulated on tolerized peripheral CD4+ cells (7, 8). It inhibits TCR signaling and promotes T cell nonresponsiveness and survival (8-10). CD5 signaling inhibits the generation of regulatory T cells but promotes the development of Th17 cells (11, 12). Within the B cell lineage, CD5 is expressed on B-1a cells, anergic B cells, and IL-10 producing regulatory B cells (13-16). Similarly to on T cells, it negatively regulates signaling through the B cell antigen receptor and supports peripheral B cell survival, anergy, and tolerance (13, 14, 16). In humans, B cells can produce an intracellularly-retained form of CD5 which lacks the signal peptide and a portion of the first SRCR domain (17). CD5 is also involved in the cellular entry of hepatitis C virus into T cells (18).

References

  1. Soldevila, G. et al. (2011) Curr. Opin. Immunol. 23:310.
  2. Huang, H.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:204.
  3. Calvo, J. et al. (1999) Tissue Antigens 54:128.
  4. Brown, M.H. and E. Lacey (2010) J. Immunol. 185:6068.
  5. Van de Velde, H. et al. (1991) Nature 351:662.
  6. Vera, J. et al. (2009) Proc. Natl. Acad. Sci. 106:1506.
  7. Azzam, H.S. et al. (1998) J. Exp. Med. 188:2301.
  8. Hawiger, D. et al. (2004) Immunity 20:695.
  9. Tarakhovsky, A. et al. (1995) Science 269:535.
  10. Friedlein, G. et al. (2007) J. Immunol. 178:6821.
  11. Ordonez-Rueda, D. et al. (2009) Eur. J. Immunol. 39:2233.
  12. de Wit, J. et al. (2011) Blood 118:6107.
  13. Bikah, G. et al. (1996) Science 274:1906.
  14. Hippen, K.L. et al. (2000) J. Exp. Med. 191:883.
  15. Yanaba, K. et al. (2008) Immunity 28:639.
  16. Gary-Gouy, H. et al. (2002) Blood 100:4537.
  17. Renaudineau, Y. et al. (2005) Blood 106:2781.
  18. Sarhan, M.A. et al. (2012) J. Virol. 86:3723.

Alternate Names

CD5

Entrez Gene IDs

921 (Human); 12507 (Mouse)

Gene Symbol

CD5

UniProt

P13379

Additional CD5 Products

Product Documents for Recombinant Mouse CD5 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD5 Protein, CF

For research use only

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