Recombinant Mouse CD97v2 Protein, CF Summary
Measured by its ability to bind rmCD55 in a functional ELISA.
When rmCD55 is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of rmCD97 that produces 50% of the optimal binding response is found to be approximately 0.15-0.5 μmL.
Gln24-His384, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD97 is a member of the LNB-TM7 protein family, which is a subfamily of the G-protein coupled receptor 2 family (1 - 3). Molecules in this family have EGF-like modules coupled to class B G protein 7-transmembrane (TM) domains by a glycosylated (mucin) stalk. Three isoforms of CD97 are produced by alternative splicing in mouse. Isoform 1, which is the longest, contains four EGF-like domains. Only the N-terminal EGF-like domain does not bind calcium. In isoform 2, which is described in this insert, does not contain this sequence that corresponds to amino acid (aa) 120 - 213 in isoform 1. Compared to isoform 1, isoform 3 lacks a 45 aa sequence between EGF-like domains 2 and 3 (4, 5). Cells known to express CD97 include monocytes, macrophages, T cells, select B cells, dendritic cells and, potentially, vascular and visceral smooth muscle cells (1, 6 - 7). CD97 is also differentially expressed on murine hematopoietic stem- and progenitor-cells (7). CD55 (decay accelerating factor), a GPI-linked cell surface molecule with short consensus repeats that regulates complement activation on cell surfaces, chondroiten sulfate and the integrin alpha 5 beta (also known as VLA-5) have been identified as cellular ligands for CD97 (7). The composition of the EGF domain region defines the ligand specificity of the different CD97 isoforms (7). The first and second EGF domains interact with CD55, whereas the fourth EGF domain binds chondroitin sulfate (7). The ligand affinity of the CD97 isoforms differs (7). While affinity for CD55 is significantly higher for the smaller isoforms, chondroitin sulfate interacts exclusively with the largest isoforms (7). It has been demonstrated that CD97 is required for neutrophil migration and host defense (8).
- McKnight, A.J. and S. Gordon (1998) J Leukoc. Biol. 63:271.
- Stacey, M. et al. (2000) Trends Biochem. Sci. 25:284.
- Stacey, M. et al. (2003) Blood 102:2916.
- Hamann, J. et al. (2000) Int. Immunol. 12:439.
- Qian, Y.-M. et al. (1999) Immunology 98:303.
- Jaspars, L.H. et al. (2001) Tissue Antigens 57:325.
- Van Pel, M. et al. (2008) Haematologica 93:1137.
- Leemans, J.C. et al. (2004) J. Immunol. 172:1125.
Citations for Recombinant Mouse CD97v2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Evaluation of Prognostic Immune Signatures in Patients with Breast, Colorectal and Pancreatic Cancer Receiving Chemotherapy
Authors: UM Vogl, L Öhler, M Rasic, JM Frischer, M Modak, J Stöckl
Anticancer Res., 2017;37(4):1947-1955.
Applications: ELISA (Standard)
Decay-accelerating factor 1 (Daf1) deficiency exacerbates xenobiotic-induced autoimmunity.
Authors: Toomey CB, Cauvi DM, Song WC
Sample Types: Whole Cells
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