Recombinant Mouse CD99-L2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
5024-CD-050
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Recombinant Mouse CD99-L2 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated rmCD99-L2 in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived mouse CD99-L2 protein
Mouse CD99-L2
(Val20 - Ala141)
Accession # NP_612182
IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa (monomer)
SDS-PAGE
60-65 kDa, reducing conditions

Product Datasheets

5024-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD99-L2

CD99 antigen-like 2 (CD99-L2) is a 45 kDa type I transmembrane glycoprotein in the CD99 family of molecules (1 - 3). Mouse CD99-L2 cDNA encodes a 214 amino acid (aa) precursor with a 23 aa predicted signal sequence, a 116 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 54 aa cytoplasmic region (4). The ECD contains no N-linked glycosylation sites, but O-linked glycosylation is likely (1, 2). A long isoform with a 23 aa insert after aa 43 within the ECD is expressed in human, and probably in mouse (2, 5). Both long and short isoforms may have minor variants missing portions of the N-terminus of the mature protein (6). The ECD of mouse CD99-L2 short isoform shares 85%, 72% and 66% aa identity with rat, human, and bovine CD99-L2, respectively (3, 5). The mouse CD99 and CD99-L2 ECDs share only 31% aa identity, but both contain three conserved acidic motifs and are thought to originate from the same ancestral gene (1, 2). The nearly ubiquitous expression of CD99-L2 is similar to that of CD99. Mouse CD99-L2 shows highest in situ hybridization signals in neurons, cortical thymocytes, ganglia, ovarian granulosa cells, testis, and kidney, and detectable protein levels in lung, thymocytes, mouse leukocytes and vascular endothelial cells (2, 7). CD99-L2 expression on endothelial cells is reported to mediate cell aggregation and neutrophil or monocyte extravasation to inflamed tissue in vivo, while CD99 mediates lymphocyte extravasation as well (3, 7).

References
  1. Park, S.H. et al. (2005) Gene 353:177.
  2. Suh, Y.H. et al. (2003) Gene 307:63.
  3. Schenkel, A.R. et al. (2007) Cell Commun. Adhes. 14:227.
  4. Entrez Accession # AAH31736.
  5. Entrez Accession # EDL26579.
  6. Entrez Accession # EDL26580 and CAM25451.
  7. Bixel, G. et al. (2007) Blood 109:5327.
Long Name
CD99 Molecule-like 2
Entrez Gene IDs
83692 (Human); 171486 (Mouse); 171485 (Rat)
Alternate Names
CD99 antigen; CD99 antigen-like 2; CD99 antigen-like protein 2; CD99 molecule-like 2; CD99B; CD99L2; CD99-L2; DKFZp761H2024; MIC2 like 1; MIC2L1; MIC2-like protein 1

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