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Recombinant Mouse CDCP1 Protein, CF

Catalog #: 9688-CU
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9688-CU-050
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CDCP1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived mouse CDCP1 protein
Arg25-Ala667, with a C-terminal 6-His tag
Accession #
Q5U462
N-terminal Sequence
Analysis
Arg25 & Ser30
Predicted Molecular Mass
73 kDa
SDS-PAGE
90-108 kDa, reducing conditions

Product Datasheets

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9688-CU

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9688-CU

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDCP1

CDCP1 (CUB-domain containing protein 1; also known as CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells (1). It is reported that this protein is over-expressed in colon and lung cancers. CDCP1 is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. The phosphorylation state of CDCP-1 has an effect on anchorage in epithelial cells (2). When unligated, CDCP1 can be proteolytically cleaved between aa 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350) (3). Over aa 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

References
  1. Hooper, J.D. et al. (2003) Oncogene 22:1783.
  2. Spassov, D.S. et al. (2013) Cancer Res. 73:1168.
  3. He, Y. et al. (2010) J. Biol. Chem. 285:26162.
Long Name
CUB Domain Containing Protein 1
Entrez Gene IDs
64866 (Human); 109332 (Mouse)
Alternate Names
CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases

Citation for Recombinant Mouse CDCP1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CDCP1 regulates retinal pigmented epithelial barrier integrity for the development of experimental autoimmune uveitis
    Authors: L Zhang, N Borjini, Y Lun, S Parab, G Enyindah-A, R Singh, BA Bell, VL Bonilha, AI Ivanov, DA Fox, RR Caspi, F Lin
    JCI Insight, 2022;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

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Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
3 Reviews

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