Recombinant Mouse CDCP1 Protein, CF
Recombinant Mouse CDCP1 Protein, CF Summary
Arg25-Ala667, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CDCP1 (CUB-domain containing protein 1; also known as CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells (1). It is reported that this protein is over-expressed in colon and lung cancers. CDCP1 is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. The phosphorylation state of CDCP-1 has an effect on anchorage in epithelial cells (2). When unligated, CDCP1 can be proteolytically cleaved between aa 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350) (3). Over aa 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.
- Hooper, J.D. et al. (2003) Oncogene 22:1783.
- Spassov, D.S. et al. (2013) Cancer Res. 73:1168.
- He, Y. et al. (2010) J. Biol. Chem. 285:26162.
Citation for Recombinant Mouse CDCP1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
CDCP1 regulates retinal pigmented epithelial barrier integrity for the development of experimental autoimmune uveitis
Authors: L Zhang, N Borjini, Y Lun, S Parab, G Enyindah-A, R Singh, BA Bell, VL Bonilha, AI Ivanov, DA Fox, RR Caspi, F Lin
JCI Insight, 2022;0(0):.
Sample Types: In Vivo
Applications: In Vivo
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