Recombinant Mouse CDCP1 Protein, CF

R&D Systems | Catalog # 9688-CU

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CDCP1 Protein (9688-CU)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q5U462
View all CDCP1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CDCP1 protein
Arg25-Ala667, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg25 & Ser30

Predicted Molecular Mass

73 kDa

SDS-PAGE

90-108 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9688-CU
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDCP1

CDCP1 (CUB-domain containing protein 1; also known as CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells (1). It is reported that this protein is over-expressed in colon and lung cancers. CDCP1 is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. The phosphorylation state of CDCP-1 has an effect on anchorage in epithelial cells (2). When unligated, CDCP1 can be proteolytically cleaved between aa 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350) (3). Over aa 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

References

  1. Hooper, J.D. et al. (2003) Oncogene 22:1783.
  2. Spassov, D.S. et al. (2013) Cancer Res. 73:1168.
  3. He, Y. et al. (2010) J. Biol. Chem. 285:26162.

Long Name

CUB Domain Containing Protein 1

Alternate Names

CD318, SIMA135

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Gene Symbol

CDCP1

UniProt

Q5U462

Additional CDCP1 Products

Product Documents for Recombinant Mouse CDCP1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CDCP1 Protein, CF

For research use only

Citations for Recombinant Mouse CDCP1 Protein, CF

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