Recombinant Mouse CL-K1/COLEC11 Protein, CF

  
  • Purity
    >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind alpha -L-Fucose. Keshi, H. et al. (2006) Microbiol. Immunol. 50(12):1001.
  • Source
    Mouse myeloma cell line, NS0-derived Gln27-Leu272, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained: Gln27 predicted
  • Predicted Molecular Mass
    27 kDa
  • SDS-PAGE
    33-38 kDa, reducing conditions
4687-CL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CL-K1/COLEC11

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the serum (4, 5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23% - 32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

  • References:
    1. Gupta, G. and A. Surolia (2007) Bioessays 29:452.
    2. van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
    3. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
    4. Keshi, H. et al. (2006) Microbiol. Immunol. 50:1001.
    5. Accession # NP_082142.
  • Long Name:
    Collectin Kidney 1
  • Entrez Gene IDs:
    78989 (Human); 71693 (Mouse)
  • Alternate Names:
    CDC28/CDC2-like kinase; CDC-like kinase 1CLKEC 2.7.12.1; CLK/STY; CLK1; CL-K1; COLEC11; dual specificity protein kinase CLK1; protein tyrosine kinase STY; STY
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB, Flow, AP MAB050R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Mouse CL-K1/COLEC11 Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information