Recombinant Mouse CL-K1/COLEC11 Protein, CF

Catalog # Availability Size / Price Qty
4687-CL-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CL-K1/COLEC11 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind alpha -L-Fucose. Keshi, H. et al. (2006) Microbiol. Immunol. 50(12):1001.
Source
Mouse myeloma cell line, NS0-derived mouse CL-K1/COLEC11 protein
Gln27-Leu272, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln27 predicted
Predicted Molecular Mass
27 kDa
SDS-PAGE
33-38 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4687-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CL-K1/COLEC11

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the serum (4, 5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23% - 32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

References
  1. Gupta, G. and A. Surolia (2007) Bioessays 29:452.
  2. van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
  3. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
  4. Keshi, H. et al. (2006) Microbiol. Immunol. 50:1001.
  5. Accession # NP_082142.
Long Name
Collectin Kidney 1
Entrez Gene IDs
78989 (Human); 71693 (Mouse)
Alternate Names
CLK1; CL-K1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb; COLEC11; collectin kidney I; Collectin kidney protein 1; collectin sub-family member 11; collectin-11; DKFZp686N1868; MGC3279

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