Recombinant Mouse Collagen XIII alpha 1 Protein, CF
Recombinant Mouse Collagen XIII alpha 1 Protein, CF Summary
When 1 x 105 cells per well are added to rmCOL-13A1 coated plate (2 μg/mL, 100 μL/well), approximately 45%-75% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Glu107-Gln565, with an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 300 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Collagen XIII alpha 1
Collagen XIII alpha 1 is an 85 kDa - 95 kDa protein in the type 2 transmembrane collagen family (1). Mature mouse Collagen XIII alpha 1 consists of a 40 amino acid (aa) cytoplasmic domain, a 19 aa transmembrane segment, and a 692 aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non‑collagenous regions (2, 3). Within comparable regions of the ECD, mouse Collagen XIII alpha 1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII alpha 1, respectively. Mouse Collagen XIII alpha 1 is extensively spliced, with some isoforms showing a tissue specific distribution (2, 4). Collagen XIII alpha 1 is widely expressed during development and in the adult (4, 5). It localizes to intercellular adherens junctions and cell-matrix focal adhesions (6, 7). Collagen XIII alpha 1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation (2, 8). Trimerization involves triple helix formation within the collagenous domains, although portions of the non‑collagenous regions can also form coiled coils (8 ‑ 10). The ECD of trimeric Collagen XIII alpha 1 is an extended rod-like structure with two flexible hinges that correspond to non‑collagenous regions (11). Collagen XIII alpha 1 clusters in cholesterol-rich domains on the plasma membrane (2, 12), and it can be cleaved from the cell surface or intracellularly by a furin-like protease (12). Collagen XIII alpha 1 binds the extracellular matrix molecules fibronectin, heparin, integrin alpha 1, nidogen‑2, and perlecan (11, 13). The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation (14).
Franzke, C.-W. et al. (2005) J. Biol. Chem. 280:4005.
Hagg, P. et al. (1998) J. Biol. Chem. 273:15590.
Pihlajaniemi, T. and M. Tamminen, 1990, J. Biol. Chem. 265:16922.
Peltonen, S. et al. (1997) DNA Cell Biol. 16:227.
Sund, M. et al. (2001) Matrix Biol. 20:215.
Peltonen, S. et al. (1999) J. Invest. Dermatol. 113:635.
Hagg, P. et al. (2001) Matrix Biol. 19:727.
Snellman, A. et al. (2000) J. Biol. Chem. 275:8936.
Latvanlehto, A. et al. (2003) J. Biol. Chem. 278:37590.
Snellman, A. et al. (2007) J. Biol. Chem. 282:14898.
Tu, H. et al. (2002) J. Biol. Chem. 277:23092.
Vaisanen, T. et al. (2006) J. Biol. Chem. 281:33352.
Nykvist, P. et al. (2000) J. Biol. Chem. 275:8255.
Vaisanen, M.-R. et al. (2006) Biochem. J. 393:43.
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