Recombinant Mouse COMP Protein, CF

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.15-0.9 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Gln20-Val755, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Gly21
  • Structure / Form
    Disulfide-linked pentamer
  • Predicted Molecular Mass
    81 kDa
  • SDS-PAGE
    85-115 kDa, reducing conditions
8958-CP
 
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Binding Activity
Recombinant Mouse COMP/Thrombospondin-5 (Catalog # 8958-CP) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.15-0.9 μg/mL.
Background: COMP/Thrombospondin-5
Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). Mouse COMP contains a non-collagenous coiled-coil domain, four EGF-like repeats, eight TSP type-3 repeats, and a globular TSP C-terminal domain (5). It shares 92% and 98% aa sequence identity with human and rat COMP, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin  alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin  alpha V beta 3 (9). COMP is up-regulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).
  • References:
    1. Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
    2. Posey, K.L. et al. (2014) Matrix Biol. 37:167.
    3. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
    4. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
    5. Fang, C. et al. (2000) J. Orthop. Res. 18:593.
    6. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
    7. Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
    8. Ozbek, S., et al. (2002) EMBO J. 21:5960.
    9. Chen, F.H., et al. (2005) J. Biol. Chem. 280:32655.
    10. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
    11. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
    12. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
    13. Kennedy, J., et al. (2005) Eur. J. Hum. Genet. 13:547.
    14. Hou, J. et al. (2000) Cell Calcium 27:309.
  • Long Name:
    Cartilage Oligomeric Matrix Protein
  • Entrez Gene IDs:
    1311 (Human); 12845 (Mouse)
  • Alternate Names:
    cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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