Recombinant Mouse Contactin-3 Protein, CF

R&D Systems | Catalog # 9800-CN

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Contactin-3 Protein (9800-CN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

AAA17403

Applications

Bioactivity
View all Contactin-3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Contactin-3 protein
Met1-Thr1003, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu20

Predicted Molecular Mass

110 kDa

SDS-PAGE

110-157 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Contactin‑3 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Amyloid precursor protein (APP) Fc Chimera that produces 50% optimal binding response is 0.02-0.1 μg/mL.

Scientific Data Images for Recombinant Mouse Contactin-3 Protein, CF

Recombinant Mouse Contactin-3 Protein Binding Activity

Recombinant Mouse Contactin-3 Protein Binding Activity

When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) is coated onto a microplate at 2 µg/mL, Recombinant Human Amyloid Precursor Protein (APP) Chimera binds with an ED50 of 0.02-0.1 μg/mL

Formulation, Preparation, and Storage

9800-CN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Contactin-3

Contactin-3 (CNTN-3), also known as brain-derived immunoglobulin superfamily protein 1 (BIG-1) and plasmacytoma-associated neuronal glycoprotein (PANG), is an axon-associated cell adhesion molecule (AxCAM). CNTN-3, together with 5 additional contactin proteins, forms the TAG-1/F3 subgroup of the Ig superfamily (1-3). Mature mouse CNTN-3 is a heavily glycosylated, 983 amino acid (aa) chain containing 6 Ig C2-like domains, 4 type III fibronectin-like domains, and a GPI anchor (1, 2). Mouse CNTN-3 shares 92% and 95% aa identity with human and rat CNTN-3, respectively. CNTN-3 is abundantly expressed in the adult brain, particularly in the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (1, 4). In mice, CNTN-3 has been associated with sensory circuitry such as the olfactory bulb and the outer segment of the retina (5, 6). Immobilized CNTN-3 promotes neurite outgrowth from rat hippocampal neurons (4). Although the physiological function of CNTN-3 is still poorly understood, experimental evidence shows that it forms both cis- and trans-complexes with protein tyrosine phosphatase gamma (PTPRG) at the cell surface (5, 6).

References

  1. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
  2. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
  3. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  4. Kamei, Y. et al. (2000) Genomics 69:113.
  5. Bouyain, S. and Watkins, D. (2009) PNAS 107:2443.
  6. Nikolaienko, R. et al. (2016) J. Biol. Chem. 291:21335.

Alternate Names

BIG-1, CNTN3, Contactin3, PANG, PCS

Entrez Gene IDs

5067 (Human); 18488 (Mouse); 54279 (Rat)

Gene Symbol

CNTN3

UniProt

AAA17403

Additional Contactin-3 Products

Product Documents for Recombinant Mouse Contactin-3 Protein, CF

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Product Specific Notices for Recombinant Mouse Contactin-3 Protein, CF

For research use only

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