Recombinant Mouse Contactin-3 Protein, CF Summary
Met1-Thr1003, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) is coated onto a microplate at 2 µg/mL, Recombinant Human Amyloid Precursor Protein (APP) Chimera binds with an ED50 of 0.02-0.1 μg/mL
Contactin-3 (CNTN-3), also known as brain-derived immunoglobulin superfamily protein 1 (BIG-1) and plasmacytoma-associated neuronal glycoprotein (PANG), is an axon-associated cell adhesion molecule (AxCAM). CNTN-3, together with 5 additional contactin proteins, forms the TAG-1/F3 subgroup of the Ig superfamily (1-3). Mature mouse CNTN-3 is a heavily glycosylated, 983 amino acid (aa) chain containing 6 Ig C2-like domains, 4 type III fibronectin-like domains, and a GPI anchor (1, 2). Mouse CNTN-3 shares 92% and 95% aa identity with human and rat CNTN-3, respectively. CNTN-3 is abundantly expressed in the adult brain, particularly in the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (1, 4). In mice, CNTN-3 has been associated with sensory circuitry such as the olfactory bulb and the outer segment of the retina (5, 6). Immobilized CNTN-3 promotes neurite outgrowth from rat hippocampal neurons (4). Although the physiological function of CNTN-3 is still poorly understood, experimental evidence shows that it forms both cis- and trans-complexes with protein tyrosine phosphatase gamma (PTPRG) at the cell surface (5, 6).
- Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
- Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
- Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
- Kamei, Y. et al. (2000) Genomics 69:113.
- Bouyain, S. and Watkins, D. (2009) PNAS 107:2443.
- Nikolaienko, R. et al. (2016) J. Biol. Chem. 291:21335.
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