Recombinant Mouse Contactin-3 Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Mouse Contactin-3 Protein Binding Activity
1 Image
Product Details
Supplemental Products

Recombinant Mouse Contactin-3 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Contactin‑3 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Amyloid precursor protein (APP) Fc Chimera that produces 50% optimal binding response is 0.02-0.1 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived mouse Contactin-3 protein
Met1-Thr1003, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
110 kDa
110-157 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse Contactin-3 Protein Binding Activity View Larger

When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) is coated onto a microplate at 2 µg/mL, Recombinant Human Amyloid Precursor Protein (APP) Chimera binds with an ED50 of 0.02-0.1 μg/mL

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Contactin-3

Contactin-3 (CNTN-3), also known as brain-derived immunoglobulin superfamily protein 1 (BIG-1) and plasmacytoma-associated neuronal glycoprotein (PANG), is an axon-associated cell adhesion molecule (AxCAM). CNTN-3, together with 5 additional contactin proteins, forms the TAG-1/F3 subgroup of the Ig superfamily (1-3). Mature mouse CNTN-3 is a heavily glycosylated, 983 amino acid (aa) chain containing 6 Ig C2-like domains, 4 type III fibronectin-like domains, and a GPI anchor (1, 2). Mouse CNTN-3 shares 92% and 95% aa identity with human and rat CNTN-3, respectively. CNTN-3 is abundantly expressed in the adult brain, particularly in the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (1, 4). In mice, CNTN-3 has been associated with sensory circuitry such as the olfactory bulb and the outer segment of the retina (5, 6). Immobilized CNTN-3 promotes neurite outgrowth from rat hippocampal neurons (4). Although the physiological function of CNTN-3 is still poorly understood, experimental evidence shows that it forms both cis- and trans­-complexes with protein tyrosine phosphatase gamma (PTPRG) at the cell surface (5, 6).

  1. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
  2. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
  3. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  4. Kamei, Y. et al. (2000) Genomics 69:113.
  5. Bouyain, S. and Watkins, D. (2009) PNAS 107:2443.
  6. Nikolaienko, R. et al. (2016) J. Biol. Chem. 291:21335.
Entrez Gene IDs
5067 (Human); 18488 (Mouse); 54279 (Rat)
Alternate Names
BIG-1; CNTN3; contactin 3 (plasmacytoma associated); Contactin3; Contactin-3; PANG; PCS


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse Contactin-3 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Contactin-3 Protein, CF and earn rewards!

Have you used Recombinant Mouse Contactin-3 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review