Recombinant Mouse CRISP-3 Protein, CF

R&D Systems | Catalog # 7056-CR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CRISP-3 Protein (7056-CR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q03402

Applications

Bioactivity
View all CRISP-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CRISP-3 protein
Gln20-Cys241, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln20 predicted (N-sequencing might be blocked)

Predicted Molecular Mass

26 kDa

SDS-PAGE

30-40 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation/survival of the 3A‑sub E human placenta cell line.

The ED50 for this effect is 1.3-5.2 μg/mL.

Formulation, Preparation, and Storage

7056-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRISP-3

CRISP-3 is one of three CRISPs (cysteine-rich secretory proteins) found in mammalian exocrine secretions and granulocytes that may play a role in innate immunity (1 - 3). CRISPs and several snake, insect, and lizard venom proteins are characterized by 16 invariant cysteine residues (4). Structurally, they consist of an N-terminal SCP domain, a hinge region, and a cysteine-rich domain (5). Human CRISP-3 is produced by salivary, pancreas, prostate, and lacrimal glands, as well as spermatozoa and mature spermatids (2, 6, 7). In mouse, CRISP-3 has not been detected in the male genital tract, although its salivary gland expression is androgen-dependent (8, 9). CRISP-3 is up‑regulated in human epithelial prostate cancer and chronic pancreatitis (10, 11). It is present as 30 kDa and 28 kDa species, corresponding to glycosylated and nonglycosylated forms (1, 3, 7, 10, 12). In human serum and seminal fluid, CRISP-3 forms high affinity noncovalent complexes with the more abundant  alpha 1B-glycoprotein and beta ‑microseminoprotein/PSP94, respectively (12, 13). Binding is mediated by the SCP domain of CRISP-3 and is independent of glycosylation (12). CRISP-3 is also expressed in pre-B cells but not in T cells or monocytes and is released from human neutrophil and eosinophil granules following cell stimulation (1, 14, 15). Mature mouse CRISP-3 shares 48% and 64% amino acid (aa) sequence identity with human and equine CRISP-3, respectively. It shares 73% and 47% aa sequence identity with mouse CRISP-1 and -2, respectively.

References

  1. Kjeldsen, L. et al. (1996) FEBS Lett. 380:246.
  2. Kratzschmar, J. et al. (1996) Eur. J. Biochem. 236:827.
  3. Udby, L. et al. (2002) J. Immunol. Meth. 263:43.
  4. Yamazaki, Y. and Morita, T. (2004) Toxicon 44:227.
  5. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
  6. Haendler, B. et al. (1999) J. Cell. Physiol. 178:371.
  7. Udby, L. et al. (2005) J. Androl. 26:333.
  8. Haendler, B. et al. (1993) Endocrinology 133:192.
  9. Haendler, B. et al. (1997) Eur. J. Biochem. 250:440.
  10. Bjartell, A. et al. (2006) Prostate 66:591.
  11. Liao, Q. et al. (2003) Histol. Histopathol. 18:425.
  12. Udby, L. et al. (2005) Biochem. Biophys. Res. Commun. 333:555.
  13. Udby, L. et al. (2004) Biochemistry 43:12877.
  14. Pfisterer, P. et al. (1996) Mol. Cell. Biol. 16:6160.
  15. Udby, L. et al. (2002) J. Leukoc. Biol. 72:462.

Long Name

Cysteine-rich Secretory Protein 3

Alternate Names

Aeg2, CRISP3, SGP28

Entrez Gene IDs

10321 (Human); 11572 (Mouse)

Gene Symbol

CRISP3

UniProt

Q03402

Additional CRISP-3 Products

Product Documents for Recombinant Mouse CRISP-3 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CRISP-3 Protein, CF

For research use only

Related Research Areas

Cancer Biomarkers
Eosinophils
Neutrophils

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