Recombinant Mouse CXADR/CAR Fc Chimera Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of human neutrophils. When 2 x 105 cells/well are added to CXADR-coated plates (10 µg/mL, 100 µL/well), approximately 40‑60% of the cells will adhere after 20 minutes at 37° C.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse CXADR
    (Leu20 - Gly237)
    Accession # P97792
    IEGRMDP Mouse IgG2A
    (Glu98 - Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu20
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    51.2 kDa (monomer)
  • SDS-PAGE
    61-65 kDa, reducing conditions
3724-NR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXADR

CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1 - 3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4 - 6). The 365 amino acid (aa) mouse CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). A PDZ binding motif at the C-terminus interacts with several cytoplasmic junctional proteins (1). The ECD of mouse CXADR shares 97%, 90%, 89%, 89% and 88% aa sequence identity with the corresponding regions of rat, human, bovine, porcine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C-terminal 15 aa shows the same expression pattern, but may show different subcellular localization (4, 8). Transcription of other splice variants has been detected, but not their translation. A secreted form identified in serum and pleural fluid can block viral infection (9).

  • References:
    1. Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
    2. Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
    3. Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
    4. Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
    5. Hotta, Y. et al. (2003) Dev. Brain Res. 143:1.
    6. Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
    7. Chen, J. et al. (2006) Circ. Res. 98:923.
    8. Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
    9. Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.
  • Long Name:
    Coxsackie and Adenovirus Receptor
  • Entrez Gene IDs:
    1525 (Human); 13052 (Mouse)
  • Alternate Names:
    CAR10Coxsackievirus B-adenovirus receptor; CAR4/6; coxsackie virus and adenovirus receptor; coxsackie virus B receptor; coxsackievirus and adenovirus receptor; CVB3 binding protein; CVB3 BP; CVB3-binding protein; CXADR; HCAR; HCVADR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid.
    Authors: Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K
    Gene Ther., 2009;16(12):1405-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Recombinant Proteins
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Recombinant Mouse IgG2A Fc, CF

BA 4460-MG 2
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