Recombinant Mouse CXCL11/I-TAC Protein

R&D Systems | Catalog # 572-MC

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse CXCL11/I-TAC Protein (572-MC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q9JHH5

Applications

Bioactivity
View all CXCL11/I-TAC Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse CXCL11/I-TAC protein
Phe22-Met100

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe22

Predicted Molecular Mass

9.1 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3.
The ED50 for this effect is 8.00‑96.0 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 572-MC
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 572-MC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL11/I-TAC

CXCL11, also known as I-TAC, SCYB9B, H174, IP-9, and beta -R1, is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to yield a 79 aa residue mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (Crg-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.

References

  1. Widney, D.P. et al. (2000) J. Immunol. 164:6322.
  2. Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
  3. Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.

Alternate Names

beta-R1, H174, I-TAC, ITAC, SCYB9B

Entrez Gene IDs

6373 (Human); 56066 (Mouse)

Gene Symbol

CXCL11

UniProt

Q9JHH5

Additional CXCL11/I-TAC Products

Product Documents for Recombinant Mouse CXCL11/I-TAC Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 572-MC (with carrier)
Download SDS 572-MC/CF (carrier free)

Product Specific Notices for Recombinant Mouse CXCL11/I-TAC Protein

For research use only

Citations for Recombinant Mouse CXCL11/I-TAC Protein

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