Recombinant Mouse Desert Hedgehog/Dhh (C23II) N-Terminus, CF

Catalog # Availability Size / Price Qty
733-DH-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Desert Hedgehog/Dhh (C23II) N-Terminus, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is <20 µg/mL.
Source
E. coli-derived mouse Desert Hedgehog/Dhh protein
Cys23-Gly198 (Cys23Ile-Ile), with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
20.1 kDa

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733-DH

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

733-DH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Desert Hedgehog/Dhh

Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling (1 - 4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal peptide, mouse Dhh shares 97% and 100% amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74% aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh) (6, 7). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (8, 9). It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation (10, 11). It also promotes the deposition of basal lamina surrounding seminiferous tubules (8). In humans, mutations of Dhh are associated with pure gonadal dysgenesis (12). Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum (13). Its upregulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis (14). Dhh is also expressed by Schwann cells and is upregulated following nerve injury (15, 16). It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (15).

References
  1. van den Brink, G.R. (2007) Physiol. Rev. 87:1343.
  2. Riobo, N.A. and D.R. Manning (2007) Biochem. J. 403:369.
  3. Porter, J.A. et al. (1995) Nature 374:363.
  4. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. 95:13630.
  5. Pathi, S. et al. (2001) Mech. Dev. 106:107.
  6. Echelard, Y. et al. (1993) Cell 75:1417.
  7. Chang, D.T. et al. (1994) Development 120:3339.
  8. Pierucci-Alves, F. et al. (2001) Biol. Reprod. 65:1392.
  9. Bitgood, M.J. et al. (1996) Curr. Biol. 6:298.
  10. Yao, H.H.-C. et al. (2002) Genes Dev. 16:1433.
  11. Park, S.Y. et al. (2007) Endocrinology 148:3704.
  12. Canto, P. et al. (2004) J. Clin. Endocrinol. 89:4480.
  13. Russell, M.C. et al. (2007) Biol. Reprod. 77:226.
  14. Chen, X. et al. (2007) Cancer Sci. 98:68.
  15. Parmantier, E. et al. (1999) Neuron 23:713.
  16. Bajestan, S.N. et al. (2006) J. Neurobiol. 66:243.
Entrez Gene IDs
50846 (Human); 13363 (Mouse)
Alternate Names
desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; Desert Hedgehog; DHH; HHG-3MGC35145

Citation for Recombinant Mouse Desert Hedgehog/Dhh (C23II) N-Terminus, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hedgehog target genes regulate lipid metabolism to drive basal cell carcinoma and medulloblastoma
    Authors: Daggubati, V;Vykunta, A;Choudhury, A;Qadeer, Z;Mirchia, K;Saulnier, O;Zakimi, N;Hines, K;Paul, M;Wang, L;Jura, N;Xu, L;Reiter, J;Taylor, M;Weiss, W;Raleigh, D;
    Research square
    Species: Mouse, Human
    Sample Types: Whole Cells
    Applications: Bioassay

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