Recombinant Mouse Dkk-1 C-Terminal Fragment Protein, CF Summary
Asp145-His272, with an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
When Recombinant Mouse LRP-6 (C-Terminal Fragment) Fc Chimera is immobilized onto a Goat anti-human IgG Fc coated plate, Recombinant Mouse Dkk‑1 C-Terminal Fragment (Catalog # 9838-DK) binds with an ED50 of 30-240 ng/mL.
related protein 1 (Dkk-1) is the founding member of the Dickkopf family of
proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2).
Mature mouse Dkk-1 is a 40 kDa secreted glycoprotein that consists of two
conserved cysteine-rich domains (CRDs), CRD1 (N-terminal) and CRD2 (C-terminal), separated by a linker region (1, 3).
Within the C-terminal fragment [amino acid (aa) 145 - 272] that includes the CRD2, mouse Dkk-1 shares 94% and 86% aa sequence identity with rat and human Dkk-1, respectively. Dkk-1 antagonizes Wnt/beta-catenin signaling, an activity for which the C-terminal CRD2 is both necessary and sufficient (4, 5), and the C-terminal fragment has been shown to interact with LRP5 or LRP6 (6). However,
crystallographic studies have shown that Dkk-1 interacts with LRP-6 as a
bipartite inhibitor, with both CRDs binding the extracellular domain of LRP-6
simultaneously (4, 7-9). Mechanistically, Dkk-1 has been shown to promote the
internalization and degradation of LRP-6, but mouse Dkk-1 may inhibit LRP-6
independently of this process (10, 11). Mice lacking Dkk-1 die at birth, lack
anterior head structures, and have limb malformations (12). Accordingly, bone
mass in mice has been found to be inversely proportional to Dkk-1 levels (13).
Reduced Dkk-1 expression causes head, limb, and vertebrae defects in mice (14).
Conversely, transgenic mice that overexpress Dkk-1 develop osteopenia (15). In
addition to bone homeostasis, Dkk-1 expression may be required for neural
differentiation of mouse embryonic stem (ES) cells (16, 17). Dkk-1 also likely has a complex role
in cancer, as it has been shown to act as a tumor suppressor and also to promote
- Glinka, A. et al. (1998) Nature 391:357.
- Niehrs, C. (2006) Oncogene 25:7469.
- Ahn, V.E. et al. (2011) Dev. Cell 21:862.
- Mao, B. et al. (2001) Nature 411:321.
- Brott, B.K. and S.Y. Sokol (2002) Mol. Cell. Biol. 22:6100.
- Kimura, H. et al. (2016) J Clin Invest. 126:7.
- Chen, S. et al. (2011) Dev. Cell 21:848.
- Bourhis, E. et al. (2011) Structure 19:1433.
- Cheng, Z. et al. (2011) Nat. Struct. Mol. Biol. 18:1204.
- Mao, B. et al. (2002) Nature 417:664.
- Semënov, M.V. et al. (2008) J. Biol. Chem. 283:21427.
- Mukhopadhyay, M. et al. (2001) Dev. Cell 1:423.
- MacDonald, B.T. et al. (2007) Bone 41:331.
- MacDonald, B.T. et al. (2004) Development 131:2543.
- Li, J. et al. (2006) Bone 39:754.
- Verani, R. et al. (2007) J. Neurochem. 100:242.
- Cajánek, L. et al. (2009) Stem Cells 27:2917.
- Menezes, M.E. et al. (2012) Int. J. Cancer 130:1477.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Mouse Dkk-1 C-Terminal Fragment Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse Dkk-1 C-Terminal Fragment Protein, CF and earn rewards!
Have you used Recombinant Mouse Dkk-1 C-Terminal Fragment Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image