Recombinant Mouse EDA-A1/Ectodysplasin A1 Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to compete with biotinylated rhEDA-A1 for binding to immobilized rhEDAR/Fc Chimera.
  • Source
    Mouse myeloma cell line, NS0-derived Ala179-Ser391, with an N-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    His
  • Structure / Form
    Homotrimer
  • Predicted Molecular Mass
    23 kDa
  • SDS-PAGE
    27-37 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
191-ED/CF
 
191-ED
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EDA-A1/Ectodysplasin A1

Ectodysplasin is a 45 kDa type II transmembrane TNF superfamily protein that is associated with X-linked hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and sweat gland development (1 - 4). The mouse EDA-A1 cDNA encodes a 41 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 329 extracellular region that contains a terminal TNF homology domain, a collagenous domain, and a stalk region (5 - 8). Within the collagenous and TNF homology domains, mouse EDA-A1 shares greater than 97% aa  sequence identity with bovine, canine, human, and rat EDA-A1. Multiple alternately spliced EDA variants have been described (4, 9). The dominant variant, EDA-A2, has a deletion of two amino acids that changes the receptor binding selectivity from EDAR to XEDAR (4, 9, 10). The collagenous domain of EDA-A1 mediates noncovalent homotrimer formation (7, 8). Shedding of the collagenous and TNF homology domains of EDA-A1 is accomplished by a furin-like protease. The released fragment maintains its trimeric state and ability to bind EDAR (11, 12). Some EDA-A1 polymorphisms found in HED patients alter the protease recognition site and prevent shedding (11). EDA-A1 is expressed in developing hair follicles, epidermis, teeth, sweat glands, salivary glands, and forebrain (8, 10, 13 - 15). It regulates ectodermal appendage formation and is critical to the patterning and morphogenesis of hair follicles, partially through the induction of Lymphotoxin beta (7, 14, 16). Receptor and ligand expression are regulated by factors involved in many aspects of tissue morphgenesis. EDA-A1 expression is induced by Wnt6, (14, 15) while the expression of EDAR is induced by Activin  beta A and inhibited by BMP-2, -4, and -7 (15, 17).

  • References:
    1. Mikkola, M.L. and I. Thesleff (2003) Cytokine Growth Factor Rev. 14:211.
    2. Botchkarev, V.A. and M.Y. Fessing (2005) J. Invest. Dermatol. 10:247.
    3. Kere, J. et al. (1996) Nat. Genet. 13:409.
    4. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
    5. Ferguson, B.M. et al. (1997) Hum. Mol. Genet. 6:1589.
    6. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. 94:13069.
    7. Ezer, S. et al. (1999) Hum. Mol. Genet. 8:2079.
    8. Mikkola, M.L. et al. (1999) Mech. Dev. 88:133.
    9. Hashimoto, T. et al. (2006) Gene 371:42.
    10. Yan, M. et al. (2000) Science 290:523.
    11. Chen, Y. et al. (2001) Proc. Natl. Acad. Sci. 98:7218.
    12. Elomaa, O. et al. (2001) Hum. Mol. Genet. 10:953.
    13. Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
    14. Laurikkala, J. et al. (2002) Development 129:2541.
    15. Laurikkala, J. et al. (2001) Dev. Biol. 229:443.
    16. Cui, C.Y. et al. (2006) Proc. Natl. Acad. Sci. 103:9142.
    17. Mou, C. et al. (2006) Proc. Natl. Acad. Sci. 103:9075.
  • Entrez Gene IDs:
    1896 (Human); 13607 (Mouse)
  • Alternate Names:
    EDAA1; EDA-A1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
    Biochem. Biophys. Res. Commun., 2009;384(3):296-300.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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